Efficacy of Calprotectin as A Marker For the Pathogenicity of Blastocystis Infection
- Conditions
- Blastocystis Infections
- Interventions
- Diagnostic Test: Fecal Calprotectin ELISA kit
- Registration Number
- NCT05724368
- Lead Sponsor
- Sohag University
- Brief Summary
Blastocystis which is commonly found in the gastro-intestinal tract, has been recognized as a non-pathogenic organism for a few decades. It has a variable distribution. A high prevalence has been reported in developing countries (22.1-100%).
In developed countries, the prevalence ranges from 0.5% to 23.1%. The high prevalence noted in developing countries is related to poor hygiene and lack of safe water and food .
- Detailed Description
Blastocystis which is commonly found in the gastro-intestinal tract, has been recognized as a non-pathogenic organism for a few decades. It has a variable distribution. A high prevalence has been reported in developing countries (22.1-100%).
In developed countries, the prevalence ranges from 0.5% to 23.1%. The high prevalence noted in developing countries is related to poor hygiene and lack of safe water and food. The pathogenicity of Blastocystis is controversial because most patients carrying this parasite are asymptomatic. Several studies have considered Blastocystis sp. as a commensal micro-organism, while other results have showed the pathogenicity of the parasite.
Calprotectin (CP), a calcium-binding protein, comprises 60% of cytosolic protein found in neutrophils. Fecal calprotectin (F-CP) concentration is an indicator of neutrophil migration into the intestinal lumen and is associated with intestinal inflammation.
Recruitment & Eligibility
- Status
- NOT_YET_RECRUITING
- Sex
- All
- Target Recruitment
- 150
- Patients from outpatient clinics, Sohag hospitals.
- Patients taking antibiotics within the previous four weeks, and anti-parasitic drugs within the previous two weeks.
Study & Design
- Study Type
- OBSERVATIONAL
- Study Design
- Not specified
- Arm && Interventions
Group Intervention Description Group 1 Fecal Calprotectin ELISA kit Patients with gastrointestinal symptoms and positive only for Blastocystis. Group 3 Fecal Calprotectin ELISA kit Healthy volunteers with neither gastrointestinal symptoms nor parasitological infections. Group 2 Fecal Calprotectin ELISA kit Patients with gastrointestinal symptoms and Blastocystis free.
- Primary Outcome Measures
Name Time Method level of fecal calprotectin 1 month Evaluation of fecal calprotectin inflammation levels of Blastocystis hominis in patients with gastrointestinal troubles as compared to patients with gastrointestinal troubles but without Blastocystis hominis
- Secondary Outcome Measures
Name Time Method