Detection of infectious virus particles using viable impactor sampling in room air surrounding children with airway infections
- Conditions
- influenzapneumonia10047438
Recruitment & Eligibility
- Status
- Recruiting
- Sex
- Not specified
- Target Recruitment
- 180
- Admitted for SARI, defined as respiratory tract infection necessitating
hospitalization.
- Tested qRT-PCR positive for RSV, HMPV, PIV and/or Influenza A+B (co-infection
allowed)
- Written Informed consent obtained
For paramyxovirus infections: Children in the age range of new-born until two
years hospitalized in the Sophia Children*s Hospital or Delft. Older children
are expected to have been infected by those viruses and thus have acquired a
pre-existing immune response which will influence the level of replication and
subsequent shedding. Therefore children > 2 years are excluded from this study.
For influenza virus infections: Children in the age range of new-born until
five years hospitalized in the Sophia Children*s Hospital or Delft. It has been
shown that primary infections with influenza viruses occur later than
infections caused by paramyxoviruses. Therefore, children until the age of 5
years are included in this study.
- no written parental informed consent, NB: Co-morbidity is not excluded since
we study real-life viral transmission routes
Study & Design
- Study Type
- Observational non invasive
- Study Design
- Not specified
- Primary Outcome Measures
Name Time Method <p>Main study parameters/endpoints: Primary end point: Time to negativity of<br /><br>viruses in the nasopharynx compared to infectious airborne virus in the room<br /><br>air. </p><br>
- Secondary Outcome Measures
Name Time Method <p>Secondary Endpoints: 1) Particle size of infectious viruses captured by viable<br /><br>impactor air sampling. Particle size will serve as a proxy for route of<br /><br>transmission. 2) Relation between clinical parameters and the load of<br /><br>infectious respiratory viruses in the nasopharynx and air<br /><br></p><br>