Gasdermin-D and Pannexin-1 in Pregnancy

Not Applicable

Completed

• Conditions: Pregnancy Complications; Maternal Obesity
• Interventions: Diagnostic Test: Biomarker measurement of pannexin-1 and gasdermin-D

• Registration Number: NCT05475951
• Lead Sponsor: Haseki Training and Research Hospital

Brief Summary

One -third of fertile women around the world are overweight or obese. This means increasing risk for both the mother and the child. Fat tissue is a factor in gestational DM development and the increase in material inflammation and oxidative stress. According to new knowledge, it is an important need to examine molecules that are not handled in new and human research in these mechanisms in fat and placenta tissues in obesity. For this purpose, the examination of the expression of gasdermin-D and pannex-1 proteins, which are shown to be produced in the cells of both tissues, is worth investigating in human fat tissue and placenta. Gasdermins and pannexins are proteins intersecting by interacting in cellular functions.

Gasdermins cause piroptosis, a type of litic proinflammatory cell death. Pannexin-1 plays in various cellular functions, including inflammation. These are examined in a small number of in vitro studies in material fat tissue and placenta, and the design of this study in people whose applications are applied is original in humans.

The status of the expressions of the gasdermin-D and pannexin-1 proteins, which will be examined for the first time in obese pregnant women's fat and placental tissues, are the subject of this research with each other and their relationship with other maternal and neonatal data.

Detailed Description

Not available

Study Timeline

• Study Start: 2021-12-01
• Status Verified: 2022-05
• Primary Completion: 2022-05-15
• Study Completion: 2022-05-30
• Study First Submitted: 2022-07-06
• Study First Submitted QC: 2022-07-25
• Last Update Submitted: 2022-07-25
• Study First Posted: 2022-07-27
• Last Update Posted: 2022-07-27

Recruitment & Eligibility

• Status: COMPLETED
• Sex: Female
• Target Recruitment: 79

Inclusion Criteria

• Being in the 20-35 age range
• Term pregnancy (37-41 weeks)
• Healthy pregnancy follow-up
• Planned cesarean section
• Having no complication in cesarean delivery
• Being a placenta with front or rear walls

Exclusion Criteria

• Intrauterine surgical procedure
• Presence of prenatal placental structural disorder
• Multiple pregnancies

Study & Design

• Study Type: INTERVENTIONAL
• Study Design: PARALLEL

Arm && Interventions

Group	Intervention	Description
Obese Pregnant Women	Biomarker measurement of pannexin-1 and gasdermin-D	The women who had term pregnancy, and had BMI values more than 30.
Overweight Pregnant Women	Biomarker measurement of pannexin-1 and gasdermin-D	The women who had term pregnancy and had BMI values between 25-30.
Normal Weight Pregnant Women	Biomarker measurement of pannexin-1 and gasdermin-D	The women who had term pregnancy and had normal BMI.

Primary Outcome Measures

Name	Time	Method
Prediction of macrosomy	7 months	Prediction of macrosomy will be assesed by meaningfull increases of studied biomarkers Gasdermin-D and Pannexin-1

Trial Locations

Locations (1)

Haseki Training and Research Hospital; 🇹🇷 Sultangazi, İstanbul, Turkey