Comparison of the effects of vitamin D and N-acetylcysteine supplementation on the expression of cellular senescence genes and inflammatory factors in the elderly with vitamin D deficiency.

Not Applicable

Recruiting

Conditions: Vitamin D deficiency.

Registration Number: IRCT20230508058120N1

Lead Sponsor: Shahid Beheshti University of Medical Sciences

Brief Summary: Not available

Detailed Description: Not available

Recruitment & Eligibility

Status: Recruiting

Sex: All

Target Recruitment: 100

Inclusion Criteria

Elderly people with a body mass index of 25-32 kg/m2
Serum vitamin D deficiency (less than 30 ng/mL)
Age over 65 years old
No chronic and acute inflammatory and infectious diseases
Not suffering from diabetes and thyroid diseases
Not suffering from Alzheimer's and dementia
Not receiving alcohol and smoking
Not receiving medication or a disorder that affects vitamin D metabolism (anticonvulsant drugs, antituberculosis drugs, glucocorticoids, HIV drugs)
Not receiving vitamin D and N-acetyl cysteine and antioxidant supplements in the last three months
Non-participation in other clinical trial studies at the same time as the present study

Exclusion Criteria

Hypercalcemia (more than 2.6 mmol/L)
Dysparathyroidism, kidney failure and stones, and long-term treatment with bisphosphonates and corticosteroids.
Consumption of less than 90% of prescribed supplements
Unwillingness to cooperate during the intervention

Study & Design

Study Type: interventional

Study Design: Not specified

Primary Outcome Measures

Name	Time	Method
Changes in p21 gene expression. Timepoint: Before and after intervention. Method of measurement: Quantitative Real-time Polymerase chain reaction.;Changes in p16 gene expression. Timepoint: Before and after intervention. Method of measurement: Quantitative Real-time Polymerase chain reaction.;Changes in TNFa gene expression. Timepoint: Before and after intervention. Method of measurement: Quantitative Real-time Polymerase chain reaction.;Changes in IL-6 gene expression. Timepoint: Before and after intervention. Method of measurement: Quantitative Real-time Polymerase chain reaction.;Changes in beta-galactosidase activity. Timepoint: Before and after intervention. Method of measurement: x-gal staining.;Serum IL-6. Timepoint: Before and after intervention. Method of measurement: ELISA KIT.;Serum TNFa. Timepoint: Before and after intervention. Method of measurement: ELISA KIT.

Secondary Outcome Measures

Name	Time	Method
R: Neutrophil to Lymphocyte Ratio. Timepoint: Before and after intervention. Method of measurement: Calculation based on complete blood count with Differential test.;C-Reactive Protein. Timepoint: Before and after intervention. Method of measurement: Elisa kit.