Bronchoalveolar Immune Response Determination

Completed

• Conditions: Extrinsic Allergic Alveolitis

• Registration Number: NCT01237145
• Lead Sponsor: Bossink, A.W.J.

Brief Summary

The purpose of this study is to establish if cells present in lung tissue during extrinsic allergic alveolitis (EAA) are able to elicit an immune response against specific antigens. The hypothesis is: cells in BAL lavage fluid from patients with EAA will be responsive to specific antigens and this response can be measured using ELISPOT.

Detailed Description

Subjects suffering from extrinsic allergic alveolitis caused by pigeons will undergo a broncho alveolar lavage. Cells obtained from this BAL will be used for ELISPOT technique with pigeon specific antigens

Study Timeline

• Study First Submitted: 2010-11-07
• Study First Submitted QC: 2010-11-08
• Study First Posted: 2010-11-09
• Study Start: 2011-09
• Primary Completion: 2012-09
• Study Completion: 2012-12
• Status Verified: 2018-01
• Last Update Submitted: 2018-01-17
• Last Update Posted: 2018-01-19

Recruitment & Eligibility

• Status: COMPLETED
• Sex: All
• Target Recruitment: 10

Inclusion Criteria

• suspicion of pigeon induced extrinsic alveolitis

Exclusion Criteria

• use of oral steroids
• unable to give informed consent
• age below 18

Study & Design

• Study Type: OBSERVATIONAL
• Study Design: Not specified

Primary Outcome Measures

Name	Time	Method
ELISPOT response in cells obtained from broncho-alveolar lavage	2 days	All elispots will be performed the same day, the end point will be available the next day

Secondary Outcome Measures

Name	Time	Method
Elispot response in cells from blood	2 days	ELispots will be performed the same day as blood collection, results will be available the next day

Trial Locations

Locations (1)

Diakonessenhuis Utrecht; 🇳🇱 Utrecht, Netherlands