Periodontal Status and Cytokines in Individuals With Non-Infectious Uveitis
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 入组人数
- 52
- 试验地点
- 1
- 主要终点
- IL-10 concentration in serum (pg/L)
研究概览
简要总结
This cross-sectional observational study aimed to evaluate periodontal clinical parameters and inflammatory cytokine levels in gingival crevicular fluid (GCF), saliva, and serum in patients with non-infectious uveitis (NIU) compared with systemically healthy individuals.
Twenty-five patients diagnosed with non-infectious uveitis and twenty-seven age- and sex-matched healthy controls were included. Clinical periodontal parameters including probing pocket depth, clinical attachment loss, plaque index, gingival index, and bleeding on probing were recorded. Biological samples including saliva, gingival crevicular fluid, and serum were collected.
Levels of TNF-α, IL-1β, IL-2, IL-6, IL-8, IL-10, and IL-17 were measured using ELISA. The study aimed to investigate whether periodontal inflammatory status and systemic cytokine profiles are associated with non-infectious uveitis.
详细描述
Non-infectious uveitis (NIU) is an immune-mediated inflammatory condition affecting the uveal tract of the eye and may be associated with systemic immune dysregulation. Periodontal disease is a chronic inflammatory disease characterized by destruction of the supporting tissues of the teeth and is known to contribute to systemic inflammatory burden through the release of cytokines and other inflammatory mediators.
Both NIU and periodontal disease involve complex immune responses and cytokine networks. However, the potential relationship between periodontal inflammation and NIU remains unclear. The present cross-sectional study was designed to investigate possible associations between periodontal clinical status and systemic and local inflammatory cytokine profiles in patients with NIU.
Participants included patients diagnosed with non-infectious uveitis and systemically healthy control individuals. Periodontal examinations were performed and biological samples including gingival crevicular fluid, saliva, and serum were collected. Levels of TNF-α, IL-1β, IL-2, IL-6, IL-8, IL-10, and IL-17 were measured using enzyme-linked immunosorbent assay (ELISA).
The objective of this study was to compare periodontal parameters and cytokine levels between NIU patients and healthy controls in order to evaluate whether periodontal inflammatory burden is associated with systemic immune alterations in individuals with non-infectious uveitis.
研究设计
- 研究类型
- Observational
- 观察模型
- Case Control
- 时间视角
- Cross Sectional
入排标准
- 年龄范围
- 18 Years 至 —（Adult, Older Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •Participants aged 18 years or older.
- •Patients diagnosed with non-infectious uveitis according to the
- •Standardization of Uveitis Nomenclature (SUN) criteria.
- •Presence of at least 20 natural teeth.
- •Individuals who agreed to participate in the study and provided informed consent.
排除标准
- •Presence of infectious uveitis.
- •Pregnancy or lactation.
- •Use of antibiotics, anti-inflammatory drugs, or immunosuppressive therapy within the previous 6 months.
- •History of periodontal treatment within the previous 6 months.
- •Presence of systemic diseases known to affect periodontal status.
- •Current smokers.
研究组 & 干预措施
Non-infectious Uveitis Group
Participants diagnosed with non-infectious uveitis who underwent periodontal examination and provided saliva, gingival crevicular fluid, and serum samples for cytokine analysis.
Healthy Control Group
Systemically healthy individuals without ocular inflammatory disease who underwent periodontal examination and biological sample collection.
结局指标
主要结局
IL-10 concentration in serum (pg/L)
时间窗: Baseline
IL-10 concentration was measured in serum samples using enzyme-linked immunosorbent assay (ELISA). Cytokine concentrations were calculated based on standard curves and expressed as picograms per liter (pg/L).
次要结局
- TNF-α concentration in gingival crevicular fluid (ng/sec)(Baseline)
- TNF-α concentration in saliva (ng/min)(Baseline)
- TNF-α concentration in serum (ng/L)(Baseline)
- IL-1β concentration in gingival crevicular fluid (pg/sec)(Baseline)
- IL-1β concentration in saliva (pg/min)(Baseline)
- IL-1β concentration in serum (pg/L)(Baseline)
- IL-2 concentration in gingival crevicular fluid (ng/sec)(Baseline)
- IL-2 concentration in saliva (ng/min)(Baseline)
- IL-2 concentration in serum (ng/L)(Baseline)
- IL-6 concentration in gingival crevicular fluid (ng/sec)(Baseline)
- IL-6 concentration in saliva (ng/min)(Baseline)
- IL-6 concentration in serum (ng/L)(Baseline)
- IL-8 concentration in gingival crevicular fluid (ng/sec)(Baseline)
- IL-8 concentration in saliva (ng/min)(Baseline)
- IL-8 concentration in serum (ng/L)(Baseline)
- IL-10 concentration in gingival crevicular fluid (pg/sec)(Baseline)
- IL-10 concentration in saliva (pg/min)(Baseline)
- IL-17 concentration in gingival crevicular fluid (ng/sec)(Baseline)
- IL-17 concentration in saliva (ng/min)(Baseline)
- IL-17 concentration in serum (ng/L)(Baseline)
研究者
Duygu Durmaz
DDS, MSc, Periodontist
Selcuk University