Development and characterization of flow able mixture of demineralized tooth matrix and injectable platelet rich fibrin graft material for socket preservation and characterization of regenerated bone

Rationale-Autogenous bone grafts are proven to be superior in comparison with other graft materials as there is no rejection. The reason for requirement of human patients in this study is, for the preparation of autogenous injectable graft using patient blood and extracted tooth (considered a biological waste)

Aim - To develop an optimal flow able mixture of demineralized tooth matrix and platelet rich fibrin graft material for socket preservation and characterize the regenerated bone

Methods- All participants willing to provide written informed consent will be recruited to the present study after fully explaining the study procedure in their own language. Extracted tooth is cleaned, soft tissue removed and dried. The tooth is demineralized partially with 0.6mol Hydrochloric acid and powdered using smart dentine grinder and washed again with Phosphate Buffered solution. The Injectable Platelet Rich Fibrin (IPRF) is prepared, mixed with the powdered tooth in 1:1 ratio and the prepared autogenous graft is placed in the extracted socket and sutured. The active control BIO-OSS is placed in the opposite extracted tooth socket.

Cone beam tomography is obtained at baseline and at the end of the study (03 months). If the participant consents for implant placement, bone tissue will be subjected for histological evaluation.