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The effects of grape seed extract intake on health

Not Applicable
Completed
Conditions
Metabolism and biological activity of oligomeric procyanidins
Not Applicable
Registration Number
ISRCTN39867491
Lead Sponsor
niversity of Milan
Brief Summary

2023 Results article in https://pubmed.ncbi.nlm.nih.gov/38104483/ (added 18/12/2023)

Detailed Description

Not available

Recruitment & Eligibility

Status
Completed
Sex
All
Target Recruitment
10
Inclusion Criteria

1. Women and men
2. Aged 18-40 years
3. BMI 18-25 kg/m²
4. Healthy
5. No smokers

Exclusion Criteria

1. Smokers
2. Allergy to grape
3. Gastrointestinal disorder
4. Liver and renal disease
5. Antibiotic treatment
5. Use of supplements

Study & Design

Study Type
Interventional
Study Design
Not specified
Primary Outcome Measures
NameTimeMethod
rinary excretion of oligomeric proanthocyanidin-derived human phenyl-?-valerolactone metabolite from Vitis vinifera, measured using high-resolution mass spectrometry techniques at baseline and at 1, 2, 4, 6, 10, 12, 14, 24 and 48 hours after the intervention and reported as absolute urinary concentration (nmoles/ml)
Secondary Outcome Measures
NameTimeMethod
1. Lipidomic, proteomic and metabolomic status measured by using omics approaches (e.g., proteomic, lipidomic, metabolomic) in the buffy coat at baseline, after 2 hours, and 7 days post-intervention. Values will be reported semi-quantitatively as fold-change.<br>2. Levels of DNA damage in the buffy coat measured by comet assay at baseline, after 2 hours and 7 days post intervention<br>3. Levels of 8-hydroxy-2'-deoxyguanosine (8-OHdG) in plasma and urine measured using ELISA kit at baseline, after 2 hours and 7 days post intervention
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