Comparative Evaluation of the Remineralizing Ability of Self-Assembling Peptide P11-4, 2% Arginine Enriched Sodium Fluoride and Functionalized Tri Calcium Phosphate Fluoride Varnishes in Treatment of White Spot Lesions (Randomized Clinical Trial and In Vitro Study)

• Test lesions will be wiped with NaOCl to remove pellicle,
• Etched for 20 s with 35% H3PO4 Etching Gel to remove mineral deposits on the tooth surface and rinsed with water in accordance with the manufacturer's instructions.
• The lesions will be dried and isolated with cotton rolls.
• The varnish will be applied and left undisturbed for 5minutes (till disappearance) to allow diffusion of self-assembly.

• The Arginine powder will be suspended at 2%.
• A 10 mL of 5% sodium fluoride varnish tube dispensed in a sterile container.
• 200 mg of Arginine will be vigorously mixed with the varnish matrix for 60 sec using a sterile microbrush.
• After dryness of the white spot lesion, the prepared varnish will be applied evenly using the micro brush.
• After application the patient will be instructed to avoid rinsing, eating soft diet and avoid hot drinks for 2 hrs.

• To limit undesirable oral ingestion, the appropriate dose for the patient will be determined with the help of the dosing guide.
• The varnish will be mixed with the brush to avoid separation of the material during storage
• However, to guarantee the best results suction will be used during application of the varnish.
• A thin uniform layer of the varnish will be applied on the lesion surface by using the supplied brush
• The applicator brush won't be moved through accumulated saliva to prevent premature setting on the bristles.
• After application the patient will be instructed to close his mouth, avoid rinsing, eating soft diet and avoid hot drinks for 4 hrs.

All patients will be encouraged to maintain good oral hygiene through regular brushing.