Geneexpression as a Marker of Embryo Viability

Completed

• Conditions: Infertility
• Interventions: Other: blastocyst biopsy

• Registration Number: NCT02399787
• Lead Sponsor: University of Aarhus

Brief Summary

The aim of the post doc project is investigate which genes regulate implantation in order to analyze specific proteins and microRNA in the spent culture media with the long-term goal of developing a non-invasive method of embryo assessment and selection. This will be achieved by conducting a targeted NGS analysis, based on list obtained from a non-published pilot study. When further validated by q-PCR, the expression of specific microRNAs known to influence the final list of genes will be analyzed in the spent culture media and the protein products of the genes recovered from the media will be quantified. The level of specific microRNAs and proteins will be related to aneuploidy and implantation potential. If the level of specific microRNA and/or proteins correlates with pregnancy, the study will form the basis for developing a clinical applicable precise method of improved embryo selection and thus improved IVF treatment.

Detailed Description

Not available

Study Timeline

• Study First Submitted: 2015-03-23
• Study First Submitted QC: 2015-03-25
• Study First Posted: 2015-03-26
• Study Start: 2015-04
• Primary Completion: 2020-05-01
• Study Completion: 2020-05-01
• Status Verified: 2020-11
• Last Update Submitted: 2020-11-06
• Last Update Posted: 2020-11-10

Recruitment & Eligibility

• Status: COMPLETED
• Sex: All
• Target Recruitment: 20

Inclusion Criteria

• >5 oocytes retrieved

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Exclusion Criteria

• endometriosis

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Study & Design

• Study Type: OBSERVATIONAL
• Study Design: Not specified

Arm && Interventions

Group	Intervention	Description
infertile patients	blastocyst biopsy	Infertile patients with more than 5 oocytes retrieved and no endometriosis

Primary Outcome Measures

Name	Time	Method
gene expression	2 years	the expression of specific microRNAs known to influence the final list of genes will be analyzed in the spent culture media and the protein products of the genes recovered from the media will be quantified. The level of specific microRNAs and proteins will be related to aneuploidy and implantation potential. If the level of specific microRNA and/or proteins correlates with pregnancy, the study will form the basis for developing a clinical applicable precise method of improved embryo selection and thus improved IVF treatment

Trial Locations

Locations (1)

The Fertility Clinic, Aarhus University Hospital, Skejby; 🇩🇰 Aarhus, Denmark