The Effect of Smoking and Non-surgical Periodontal Treatment on Gingival Crevicular Fluid Cyclophilin A and EMMPRIN Levels

Not Applicable

Completed

• Conditions: Periodontitis; Smoking; Non-surgical Periodontal Treatment
• Interventions: Procedure: non-surgical periodontal treatment

• Registration Number: NCT04430764
• Lead Sponsor: Ege University

Brief Summary

This study aimed to investigate the effect of smoking and non-surgical periodontal treatment on gingival crevicular fluid CyPA and EMMPRIN levels in patients with periodontitis. Twenty-eight patients with periodontitis (14 non-smoker and 14 smoker) and 30 control subjects (15 non-smoker and 15 smoker) were included into the study. Non-surgical periodontal treatment was applied to all periodontitis patients. Periodontal parameters and GCF CyPA and EMMPRIN levels were evaluated during 3 months following non-surgical periodontal treatment. GCF CyPA and EMMPRIN levels were analyzed by ELISA.

Detailed Description

Not available

Study Timeline

• Study Start: 2014-10
• Primary Completion: 2015-08
• Study Completion: 2015-08
• Status Verified: 2020-06
• Study First Submitted: 2020-06-09
• Study First Submitted QC: 2020-06-11
• Last Update Submitted: 2020-06-11
• Study First Posted: 2020-06-12
• Last Update Posted: 2020-06-12

Recruitment & Eligibility

• Status: COMPLETED
• Sex: All
• Target Recruitment: 58

Inclusion Criteria

• generalized periodontitis with stage III-IV & Grade B
• no systemic and periodontal diseases
• smokers (minimum 10 cigarettes per day for at least ten years)
• non-smokers (never smoked)

Exclusion Criteria

• Systemic diseases
• Infectious diseases
• Pregnancy or lactating
• Having periodontal treatment during the last 6 months
• Having antibiotic treatment within the past 3 months
• Former smokers

Study & Design

• Study Type: INTERVENTIONAL
• Study Design: PARALLEL

Arm && Interventions

Group	Intervention	Description
smoker	non-surgical periodontal treatment	-
non-smoker	non-surgical periodontal treatment	-

Primary Outcome Measures

Name	Time	Method
Change in gingival crevicular fluid EMMPRIN total amounts	Baseline, 1 week, 1., 3. months	GCF EMMPRIN levels were analyzed by ELISA
Change in gingival crevicular fluid CypA total amounts	Baseline, 1 week, 1., 3. months	GCF CyPA levels were analyzed by ELISA

Secondary Outcome Measures

Name	Time	Method
Change in bleeding on probing	Baseline, 1., 3. months	Bleeding on probing was evaluated the presence or absense following bleeding upon probing.
Change in probing depth	Baseline, 1., 3. months	Probing depth was measured with Williams periodontal probe.