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Patient-derived Stem Cell Models of Anorexia Nervosa

Active, not recruiting
Conditions
Anorexia Nervosa
Interventions
Other: observational
Registration Number
NCT06259500
Lead Sponsor
Karolinska Institutet
Brief Summary

Experimental and genetic data, as well as brain imaging, support a role of the hypothalamic Arcuate nucleus neurons and their communication with surrounding microglia in anorectic conditions, but it has until recently not been possible to explore these cells at a molecular level in anorexia nervosa (AN) patients. This attributed to the obvious lack of valid tissue and non-invasive imaging techniques of high enough resolution. Stem cell models have evolved as a useful tool for the exploration of other neuropsychiatric disorders with a comparably high genetic contribution as AN. The investigators will here use patient-derived induced pluripotent stem cells (iPSCs) to profile Arc neurons and peripheral blood mononuclear cell (PBMC) derived microglia in AN, thus defining molecules to explore as drug targets.

Detailed Description

Not available

Recruitment & Eligibility

Status
ACTIVE_NOT_RECRUITING
Sex
Female
Target Recruitment
20
Inclusion Criteria

Not provided

Exclusion Criteria

Not provided

Study & Design

Study Type
OBSERVATIONAL
Study Design
Not specified
Arm && Interventions
GroupInterventionDescription
anorexia nervosaobservational-
healthy controlsobservational-
Primary Outcome Measures
NameTimeMethod
Arcuate neuron function and transcriptomic profiling2026

Electrical activity, transmitter release and whole transcriptomic profiling of iPSC derived Arcuate neurons, anorexia nervosa vs healthy controls

Microglia function and transcriptomic profiling2025

phagocytic and immune activation capacity and whole transcriptomic profiling, anorexia nervosa vs healthy controls

Secondary Outcome Measures
NameTimeMethod

Trial Locations

Locations (1)

Karolinska Institutet

🇸🇪

Stockholm, Sweden

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