Development Of a Novel Point-Of-Care- Testing Device For Rapid Diagnosis And Evaluation Of In-Vitro Therapeutic Potential Of IgY antibody For Vulvovginal Candidiasis At a Tertiary Care Hospital.
试验速览
- 阶段
- 不适用
- 状态
- 招募中
- 发起方
- 入组人数
- 100
- 试验地点
- 1
- 主要终点
- Novel point of care testing device based on the principle of lateral flow immunoassay and colorimetric method to diagnose vulvovaginal candidiasis rapidly and accurately will be developed
研究概览
简要总结
Aim:
The aim of the study is to develop novel point of care testing device to diagnose vulvovaginal candidiasis and evaluate the therapeutic approach of IgY against drug- resistant Candida species.
Objective:
•To evaluate the enzymatic activity of Candida species by colorimetric method
•To extract the Immunoglobulin Y (IgY) from Hen’s egg yolk by Polyethylene glycol precipitation method
•To evaluate the potential activity of IgY against Candida biofilm produced by drug resistant Candida species by MTT assay (tetrazolium salt assay)
研究设计
- 研究类型
- Observational
入排标准
- 年龄范围
- 18.00 Year(s) 至 55.00 Year(s)（—）
- 性别
- Female
入选标准
- •The study includes Candida isolates from vaginal swabs of sexually active women aged above 18 years from Obstetrics and Gynaecology that are routinely sent to the Microbiology laboratory.
排除标准
- •Age less than 18 years.
- •Patients on any antifungal therapy 6 weeks prior to sample collection were excluded from the study Patients other than Candida infection.
结局指标
主要结局
Novel point of care testing device based on the principle of lateral flow immunoassay and colorimetric method to diagnose vulvovaginal candidiasis rapidly and accurately will be developed
时间窗: October 2024- March 2026
次要结局
- Therapeutic potential of IgY antibodies to inhibit biofilm formation OF DRUG RESISTANT Candida species in-vitro will be identified
研究者
DEEKSHA P
SRM Medical college