Clinical evaluation of the time effect relationship of auriculoacupuncture at point zero on autonomic function among healthy volunteers

Auriculotherapy (Part of Acupuncture) has been utilized in Traditional Chinese Medicine (TCM) for both diagnosis and treatment. According to TCM, the ear is believed to be directly connected with the 12 meridians.Dr. Paul Nogier introduced an ear diagrammatic chart in 1957, representing an inverted fetus, which was subsequently approved by the World Health Organization (WHO).In 2009, Dr.Romoli and Mazzoni updated this chart into a sectogram, incorporating auriculotherapy points that extend outward from the root of the helix, known as Point Zero. The practice of acupuncture depends on several factors like type of needle, frequency, technique, manual acupuncture, electrical acupuncture, disease and particularly duration of needle retention makes it difficult for clinical practice. The duration of acupuncture treatment has not been paid attention on clinical research so  this study focuses on investigating the effects of needle retention period on auriculotherapy at Point Zero. Healthy volunteers of age group between 18- 40 years with BMI (18-25 kg/m2). After screening of healthy volunteers ,they will be divided into 3 different groups, each group will have n=30 subjects called Arm 1, Arm 2, Arm 3. Participants of Arm 1(n=30) will receive acupuncture at Point zero with Press needle(3×3mm) for a duration of 5 minutes, Arm 2(n=30) participants will receive the same procedure for 30 minutes and participants of Arm 3 will receive the same procedure for 60(n=30) minutes.  As a primary outcome, HRV will be assessed on baseline and after 5/30/60 minutes. As a secondary outcome, PEFM ( Peak Exploratory Flow Meter) will be assessed on baseline and after 5/30/60 minutes.