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Selenium and Prostate Cancer: Clinical Trial on Availability to Prostate Tissue and Effects on Gene Expression

Not Applicable
Completed
Conditions
Prostatic Neoplasms
Prostate Cancer
Interventions
Dietary Supplement: Selenium
Registration Number
NCT00446901
Lead Sponsor
Wageningen University
Brief Summary

The aim of this study is to determine whether selenium supplementation leads to changes in selenium levels and gene expression profiles in prostate tissue.

Detailed Description

Rationale: Prostate cancer is a frequently observed malignancy in men, especially in elderly men. Besides diagnosis and treatment, also prevention of prostate cancer is an important point of interest to reduce the incidence and mortality of prostate cancer. Selenium is considered to be a promising chemopreventive agent for prostate cancer. Exact mechanisms of chemoprevention by selenium are not fully understood. However, it is expected that selenium (among other effects) directly affects gene expression in the prostate.

Objective: The aim of this study is to get insight into bioavailability of selenium in prostate tissue and changes of gene expression profiles that might be responsible for selenium-induced chemoprevention. To meet this objective, the relationship between dietary selenium intake and changes in gene expression profiles, tissue selenium levels and blood flow in prostate tissue will be examined.

Study design: The present study is designed as a double-blind, randomized and placebo-controlled intervention trial. Blood samples, toenails, questionnaires, MR images and surgical specimens will be collected to examine effects of selenium supplementation.

Study population: The study population will consist of 60 men, diagnosed with prostate cancer and scheduled for radical prostatectomy. Written informed consent will be obtained from each participant.

Intervention: Participants will receive 300 ug selenium / day or a placebo during 5 weeks prior to radical prostatectomy. Selenium will be supplemented in the form of selenized yeast tablets (SelenoPrecise, Pharma Nord).

Main study parameters: Levels of selenium in prostate tissue and changes in prostate gene expression profiles of participants supplemented with selenium or placebo, compared before and after the short intervention period, will be considered as the main parameters of the present study. Besides gene expression profiles in prostate tissue, also gene expression profiles of peripheral mononuclear cells (PBMC), levels of selenium in blood and toenails and blood flow and permeability of blood vessels of prostate tissue will be analyzed.

Recruitment & Eligibility

Status
COMPLETED
Sex
Male
Target Recruitment
60
Inclusion Criteria
  • male
  • biopsy proven prostate cancer
  • scheduled for radical prostatectomy
Exclusion Criteria
  • liver diseases (e.g. hepatitis)
  • kidney diseases
  • inflammatory bowel diseases
  • use of dietary supplements containing selenium
  • adjuvant therapy for prostate cancer (e.g. hormonal therapy, HIFU)
  • previously or concurrent diagnosed with cancer, other than prostate cancer

Study & Design

Study Type
INTERVENTIONAL
Study Design
PARALLEL
Arm && Interventions
GroupInterventionDescription
PlaceboSeleniumPlacebo
Selenium (selenized yeast)SeleniumSelenium (selenized yeast) tablets, 300 ug/day
Primary Outcome Measures
NameTimeMethod
selenium levels in prostate tissueafter 5 weeks of intervention with selenium or placebo
changes in gene expression profiles in prostate tissueafter 5 weeks of intervention with selenium or placebo
Secondary Outcome Measures
NameTimeMethod
changes in blood flow, vessel permeability and exocrine functionalityafter 5 weeks of intervention with selenium or placebo
changes in gene expression profiles in blood cellsafter 5 weeks of intervention with selenium or placebo

Trial Locations

Locations (2)

University Medical Centre St Radboud

🇳🇱

Nijmegen, Gelderland, Netherlands

Wageningen University

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Wageningen, Gelderland, Netherlands

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