Study of Pomegranate Juice in the Modulation of Oxidative Stress Markers in Endurance-based Athletes
Trial Snapshot
- Phase
- Phase 1
- Status
- Completed
- Enrollment
- 20
- Primary Endpoint
- Protein carbonyls levels changes in plasma
Study Overview
Brief Summary
The purpose of this study is to evaluate modifications of plasma protein carbonyls and malondialdehyde levels in endurance-based athletes after three weeks of pomegranate juice consumption.
Study Design
- Study Type
- Interventional
- Allocation
- Randomized
- Intervention Model
- Parallel
- Primary Purpose
- Treatment
- Masking
- Triple (Participant, Investigator, Outcomes Assessor)
Eligibility Criteria
- Ages
- 18 Years to 50 Years (Adult)
- Sex
- Male
- Accepts Healthy Volunteers
- Yes
Inclusion Criteria
- •Perform specific endurance training sessions
- •More than 1 hour per session and more than 3 sessions per week
- •Have participated recently in a half marathon or similar event
Exclusion Criteria
- •Intake of antioxidant or anti-inflammatory supplementation
- •Present a chronic disorder
- •Non-smoker
- •No-alcoholic beverage consumption
Arms & Interventions
Control
Arm that followed all the protocols with the exception of the pomegranate juice intake.
Pomegranate Juice
Arm that followed all the protocols and the pomegranate juice intake.
Intervention: Pomegranate juice (Dietary Supplement)
Pomegranate Juice Dilution
Arm that followed all the protocols and the pomegranate juice dilution (1:1) intake.
Intervention: Pomegranate juice (Dietary Supplement)
Outcomes
Primary Outcomes
Protein carbonyls levels changes in plasma
Time Frame: day 0 and 22
Protein carbonyl derivatives were determined using an adaptation of the colorimetric method published in Levine et al. (1994) based in the formation of a stable 2,4-dinitrophenyl (DNP) hydrazone product after the addition of 2,4-dinitrophenylhydrazine (DNPH) to tha plasma. Protein carbonyls were calculated as nmoles·mg-1 protein
Malondialdehyde levels changes in plasma
Time Frame: day 0 and 22
Malondialdehyde was determined by high pressure liquid chromatography (HPLC) with fluorescence detection according to the method described by Laporta et al. (2007). Malondialdehyde levels were calculated as nmoles·g-1 protein
Secondary Outcomes
- Blood biochemical parameters changes in plasma(day 0 and 22)
- C-reactive protein levels changes in plasma(day 0 and 22)
- Soluble E-selectin levels changes in plasma(day 0 and 22)
Investigators
Néstor Vicente-Salar
PhD
Universidad Miguel Hernandez de Elche