Study of Pomegranate Juice in the Modulation of Oxidative Stress Markers in Endurance-based Athletes
- Conditions
- Oxidative Stress
- Interventions
- Dietary Supplement: Pomegranate juice
- Registration Number
- NCT02293486
- Lead Sponsor
- Universidad Miguel Hernandez de Elche
- Brief Summary
The purpose of this study is to evaluate modifications of plasma protein carbonyls and malondialdehyde levels in endurance-based athletes after three weeks of pomegranate juice consumption.
- Detailed Description
Not available
Recruitment & Eligibility
- Status
- COMPLETED
- Sex
- Male
- Target Recruitment
- 20
- Perform specific endurance training sessions
- More than 1 hour per session and more than 3 sessions per week
- Have participated recently in a half marathon or similar event
- Intake of antioxidant or anti-inflammatory supplementation
- Present a chronic disorder
- Non-smoker
- No-alcoholic beverage consumption
Study & Design
- Study Type
- INTERVENTIONAL
- Study Design
- PARALLEL
- Arm && Interventions
Group Intervention Description Pomegranate Juice Dilution Pomegranate juice Arm that followed all the protocols and the pomegranate juice dilution (1:1) intake. Pomegranate Juice Pomegranate juice Arm that followed all the protocols and the pomegranate juice intake.
- Primary Outcome Measures
Name Time Method Protein carbonyls levels changes in plasma day 0 and 22 Protein carbonyl derivatives were determined using an adaptation of the colorimetric method published in Levine et al. (1994) based in the formation of a stable 2,4-dinitrophenyl (DNP) hydrazone product after the addition of 2,4-dinitrophenylhydrazine (DNPH) to tha plasma. Protein carbonyls were calculated as nmoles·mg-1 protein
Malondialdehyde levels changes in plasma day 0 and 22 Malondialdehyde was determined by high pressure liquid chromatography (HPLC) with fluorescence detection according to the method described by Laporta et al. (2007). Malondialdehyde levels were calculated as nmoles·g-1 protein
- Secondary Outcome Measures
Name Time Method Blood biochemical parameters changes in plasma day 0 and 22 Blood biochemical parameters is a composite outcome measure consisting in circulating plasma glucose (mg·dL-1), HDL-cholesterol (mg·dL-1), triglycerides (mg·dL-1), ferritin (ng·mL-1), lactate (mg·dL-1), sodium (mEq·L-1), potassium (mEq·L-1), creatine kinase (U·L-1), aspartate aminotransferase (U·L-1) and alanine aminotransferase (U·L-1) were analyzed through biochemistry methods.
C-reactive protein levels changes in plasma day 0 and 22 Determination by immunoassay of C-reactive protein (ng·mL-1) levels in plasma.
Soluble E-selectin levels changes in plasma day 0 and 22 Determination by immunoassay of soluble E-selectin (ng·mL-1) levels in plasma.