The Influence of Different Concentrations of Glucose in Culture Media on Fertilization and Embryo Quality

Not Applicable

• Conditions: Fertility
• Interventions: Other: Universal IVF Medium + ISM1 Medium

• Registration Number: NCT00606073
• Lead Sponsor: Hillel Yaffe Medical Center

Brief Summary

The aim of this study is to examine whether incubation of human oocytes until pronuclear stage, in different culture media containing high and low concentrations of glucose affect fertilization differently, not only in standard IVF cycles, but also after intracytoplasmatic sperm injection.In addition, we wish to determine whether exposure of oocytes to different concentrations of glucose until pronuclear stage has further effect on embryo quality and development until the blastocyst stage.

Detailed Description

Not available

Study Timeline

• Status Verified: 2008-01
• Study First Submitted: 2008-01-21
• Study First Submitted QC: 2008-01-21
• Last Update Submitted: 2008-01-21
• Study Start: 2008-02
• Study First Posted: 2008-02-01
• Last Update Posted: 2008-02-01
• Primary Completion: 2009-08

Recruitment & Eligibility

• Status: UNKNOWN
• Sex: All
• Target Recruitment: 100

Inclusion Criteria

• Patients undergoing IVF treatment in our clinic during study period

Exclusion Criteria

• Females older than 40 years
• Patients with less than 6 retrieved oocytes
• Male patients older than 50 years old
• Patients after testis biopsies

Study & Design

• Study Type: INTERVENTIONAL
• Study Design: PARALLEL

Arm && Interventions

Group	Intervention	Description
III	Universal IVF Medium + ISM1 Medium	ICSI Procedure - IVF Medium
IV	Universal IVF Medium + ISM1 Medium	ICSI Procedure - ISM1 Medium
I	Universal IVF Medium + ISM1 Medium	IVF Procedure-IVF Medium
II	Universal IVF Medium + ISM1 Medium	IVF Procedure - ISM1 Medium

Primary Outcome Measures

Name	Time	Method
Fertilization rate and embryo quality	6 days