Hookworm Immune Regulation Project

Not Applicable

• Conditions: Hookworm Infection
• Interventions: Other: Blood and feces sampling

• Registration Number: NCT02262403
• Lead Sponsor: Brugmann University Hospital

Brief Summary

The main objective of this study is to characterize the regulatory immune response induced by hookworm in an infected Vietnamese rural population from the periphery of HCM, evolution after infection treatment and during potential naturally reinfection.

Detailed Description

Population: 20 healthy adults (18-65 years) infected and non infected by Hookworm will be recruited and treated according to Good Clinical Practice recommendations.

Allergy will be excluded by skin prick tests. Amount and phenotype of Treg will be explored at several time points. Subsequent culture with environmental antigen will be performed on cryopreserved cells.

Study Timeline

• Study Start: 2014-08-01
• Study First Submitted: 2014-09-16
• Study First Submitted QC: 2014-10-08
• Study First Posted: 2014-10-13
• Status Verified: 2021-02
• Last Update Submitted: 2021-02-18
• Last Update Posted: 2021-02-21
• Primary Completion: 2021-12
• Study Completion: 2021-12

Recruitment & Eligibility

• Status: UNKNOWN
• Sex: All
• Target Recruitment: 40

Inclusion Criteria

• 18-65 years old adults in good health status
• To live in rural regions at risk of soil transmitted helminthes
• Hookworm infection (infected group)
• Uninfected by Hookworms (control group)

Exclusion Criteria

• Pregnant woman
• Positive allergic history
• Auto-immune and/or HIV disease
• Antihelminthics drug in the last 6 months and other current parasitic infection

Study & Design

• Study Type: INTERVENTIONAL
• Study Design: PARALLEL

Arm && Interventions

Group	Intervention	Description
Hookworm infected	Blood and feces sampling	The amount, phenotype and function of Treg will be explored at several time points. Cultures with environmental antigen will be subsequently performed.
Non infected (hookworms) healthy subjects	Blood and feces sampling	All the tests done in the experimental hookworm infected group will be also done in the comparator non infected group.

Primary Outcome Measures

Name	Time	Method
Amount and phenotype of Treg	0-12 weeks	Four colors flow cytometry Treg measurements and phenotyping (FACSCantoII).

Trial Locations

Locations (1)

Pham Ngoc Thach University of Medecine; 🇻🇳 Ho Chi Minh City, Distict 10, Vietnam