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Clinical Trials/EUCTR2016-002790-35-FR
EUCTR2016-002790-35-FR
Active, not recruiting
Phase 1

Phase I/II ex vivo gene therapy clinical trial for RDEB using autologous skin equivalent grafts genetically corrected with a COL7A1-encoding SIN retroviral vector - EBGraft

INSERM0 sites3 target enrollmentJanuary 17, 2018

Overview

Phase
Phase 1
Intervention
Not specified
Conditions
The trial aims to treat the recessive dystrophic epidermolysis bullosa (RDEB) by grafting one to three subjects with RDEB with autologous COL7A1-modified skin equivalents, using SIN-RV encoding COL7A1 cDNA.
Sponsor
INSERM
Enrollment
3
Status
Active, not recruiting
Last Updated
6 years ago

Overview

Brief Summary

No summary available.

Registry
who.int
Start Date
January 17, 2018
End Date
TBD
Last Updated
6 years ago
Study Type
Interventional clinical trial of medicinal product

Investigators

Sponsor
INSERM

Eligibility Criteria

Inclusion Criteria

  • 1\. \= 18 year\-old
  • 2\. Clinical and molecular diagnosis of RDEB with confirmed bi\-allelic COL7A1 mutations
  • 3\. Significantly reduced staining of C7 on skin biopsy, measured by immunofluorescence microscopy (IF)
  • 4\. A reduced number of or morphologically abnormal anchoring fibrils confirmed by TEM
  • 5\. Presence of non\-collagenous\-1 domain (NC\-1\) of C7 on skin biopsy, measured by immunofluorescence microscopy (IF) and/or Western blot analysis
  • 6\. Presence of \=100cm2 of blistered and/or erosive skin areas including chronic wounds suitable for skin grafting
  • 7\. Ability to undergo anaesthesia for grafting procedures
  • 8\. Subjects aged \= 18years, willing and able to give informed consent
  • Are the trial subjects under 18? no
  • Number of subjects for this age range:

Exclusion Criteria

  • 1\. Recipients of other investigational medicinal products within 6 months prior to enrolment into this study
  • 2\. Past medical history of biopsy proven skin malignancy
  • 3\. Immunotherapy including oral corticosteroids (Prednisolone \>1mg/kg) for more than one week (intranasal and topical preparations are permitted) or chemotherapy within 60 days of enrolment into this study
  • 4\. Known allergy to any of the constituents of the investigational medicinal product (IMP) including Penicillin
  • 5\. Subjects with BOTH:
  • positive serum antibodies to C7 confirmed by ELISA and
  • positive IIF with binding to the base of salt split skin and/or
  • positive Western blot
  • 6\. Positive results for HIV, Hepatitis BsAg, Hepatitis BcAb, Hepatitis C IgG, HTLV1\&2 or Syphilis serology
  • 7\. Clinically significant medical, psychological or laboratory abnormalities limiting the ability of the subject to travel to the trial site(s) and to undergo grafting and follow\-up procedures, as determined by the Investigator

Outcomes

Primary Outcomes

Not specified

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