Oocyte Survival After Vitrification in an Open or Closed Device With Closed Storage.

Not Applicable

Completed

• Conditions: Survival; Embryo Quality
• Interventions: Device: Cryotop SC device; Device: CBSvit HS device

• Registration Number: NCT01952184
• Lead Sponsor: Universitair Ziekenhuis Brussel

Brief Summary

The survival rate (and embryo quality) of donor oocytes with two commercially available devices.

Detailed Description

Donor sibling oocytes will be vitrified with two commercially available devices. The first device is closed vitrification with closed storage and the second device is open vitrification with closed storage. Oocyte acceptors receive oocytes from both devices. With the increase in cooling rate generated by the open vitrification (second device), we expect to get an increase in survival rate of 10%. Besides this, embryo quality will be assessed with oocytes obtained from both devices.

Study Timeline

• Study Start: 2013-09
• Study First Submitted: 2013-09-24
• Study First Submitted QC: 2013-09-26
• Study First Posted: 2013-09-27
• Primary Completion: 2015-07
• Study Completion: 2015-07
• Status Verified: 2015-12
• Last Update Submitted: 2015-12-02
• Last Update Posted: 2015-12-03

Recruitment & Eligibility

• Status: COMPLETED
• Sex: Female
• Target Recruitment: 48

Inclusion Criteria

• Donor: ≤36 years old on day of oocyte retrieval
• Donor: ≥ 6 mature oocytes

Exclusion Criteria

• Donor: < 6 mature oocytes

Study & Design

• Study Type: INTERVENTIONAL
• Study Design: PARALLEL

Arm && Interventions

Group	Intervention	Description
open vitrification with closed storage	Cryotop SC device	Half of the oocytes of every donor will be vitrified with the Cryotop closed system (Cryotop SC).
closed vitrification with closed storage	CBSvit HS device	Half of the oocytes of every donor will be vitrified with the Cryo Bio Systems High Security vitrification (CBSvit HS) device; the standard device in our lab.

Primary Outcome Measures

Name	Time	Method
Survival rate	up to 6 days after every warming cycle	The purpose is to assess the survival rate of donor oocytes with the two vitrification devices.

Secondary Outcome Measures

Name	Time	Method
Embryo development	up to 6 days after every warming cycle	The purpose is to analyse the pre-implantation development of donor oocytes vitrified with two different devices.

Trial Locations

Locations (1)

Universitair Ziekenhuis Brussel; 🇧🇪 Jette, Brussels, Belgium