The effect of upper gastrointestinal surgery on the acute systemic inflammatory response

Completed

• Conditions: immune response; inflammatory response; 10017969; 10003816; 10017998

• Registration Number: NL-OMON46239
• Lead Sponsor: Catharina-ziekenhuis

Brief Summary

Not available

Detailed Description

Not available

Study Timeline

• Last Update Posted: 23 April 2024

Recruitment & Eligibility

• Status: Completed
• Sex: Not specified
• Target Recruitment: 50

Inclusion Criteria

- Subjects who undergo elective surgery in which the splenic artery is exposed as part of the procedure
- Written informed consent
- Older than 18 years

Exclusion Criteria

- Previous splenectomy
- Use of steroids 4 weeks prior to inclusion
- Use of immunosuppresive agents (e.g. methotrexate, biologicals)

Study & Design

• Study Type: Observational invasive
• Study Design: Not specified

Primary Outcome Measures

Name	Time	Method
<p>Mapping of the systemic inflammatory response, as measured by means of<br /><br>pro-inflammatory cytokines, protein and transcript expression. Cytometric bead<br /><br>array will be used to identify important pro-inflammatory cytokines, amongst<br /><br>which most likely TNF-alpha, interferon gamma and interleukins such as IL-1,<br /><br>IL-6, IL-8, and IL-10. These cytokines will be analyzed in more detail using<br /><br>assays such as Enzyme Linked ImmunoSorbent Assay (ELISA) and by means of<br /><br>Polymerase Chain Reaction (PCR) for transcript expression.</p><br>

Secondary Outcome Measures

Name	Time	Method
<p>- the effect of ex vivo lipopolysaccharide (LPS) stimulations on the Toll-like<br /><br>receptor (TLR) responses of monocytes in these patients.<br /><br>- the effect on the local pro- and anti-inflammatory responses in splenic<br /><br>tissue in patients undergoing pancreaticosplenectomy (RAMPS procedure).<br /><br>- the presence of nerve fibres in the gastrosplenic ligament in patients<br /><br>undergoing pancreaticosplenectomy or esophagectomy.</p><br>