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Semen Analysis Parameters Following Pfizer's COVID-19 Vaccine

Conditions
Covid19
Fertility Issues
Interventions
Other: Sperm collection
Registration Number
NCT04778033
Lead Sponsor
Sheba Medical Center
Brief Summary

In this study the investigators will explore the effect of the BNT162b2 COVID-19 Vaccine on semen analysis parameters among 75 fertile men. Fertile men will be regarded as such if they previously successfully impregnated their partners without the use of artificial reproductive technology, or men who previously had a normal sperm analysis exam by WHO criteria (2010). Those recruited will supply a single sperm sample 1-2 months following the second dose of the vaccination. In addition, the participants will fill a brief questionnaire with information regarding their medical and reproductive history. The investigators will examine the macroscopic and microscopic properties of the semen samples and compare them to the WHO semen analysis reference range in an attempt to ascertain whether the vaccine influences sperm parameters.

Detailed Description

The 2019 novel coronavirus disease (COVID-19) is a highly infectious respiratory tract disease which was first reported in Wuhan, Hubei Province, China in Dec 2019, but has since spread globally. By march 2020 it was declared by WHO as a public health emergency pandemic and has thus far affected tens of millions worldwide. The implications of COVID 19 infection on male fertility were examined in a previous study, which found decreased sperm concentration in recovering patients compared with controls.

The newly available mRNA COVID 19 vaccine by Pfizer was recently evaluated in a large multicenter placebo controlled RCT. The vaccine is comprised of BNT162b2, a lipid nanoparticle formulated nucleoside-modified RNA (modRNA) encoding the SARS-CoV2 full length spike protein, modified by two Proline mutations. The two 20 µg doses were shown to elicit high SARS-COV2 neutralizing antibody titers alongside high antigen specific CD8+ and Th1 type CD4+ T cell response. It was subsequently shown to be 95% effective in preventing COVID-19 infection a week following the second dose with a favorable safety profile in a 2-month median following up time.

Unfounded claims in the popular media linked a possible correlation between the COVID-19 vaccine and potential female\\male infertility. Currently. there is no information in the medical literature which examined semen analysis parameters following the COVD-19 vaccine.

In this study the investigators will explore the effect of the BNT162b2 COVID-19 Vaccine on semen analysis parameters among 75 fertile men. Fertile men will be regarded as such if they previously successfully impregnated their partners without the use of artificial reproductive technology, or men who previously had a normal sperm analysis exam by WHO criteria (2010). Those recruited will supply a single sperm sample 1-2 months following the second dose of the vaccination. In addition, the participants will fill a brief questionnaire with information regarding their medical and reproductive history. The investigators will examine the macroscopic and microscopic properties of the semen samples and compare them to the WHO semen analysis reference range in an attempt to ascertain whether the vaccine influences sperm parameters.

Recruitment & Eligibility

Status
UNKNOWN
Sex
Male
Target Recruitment
75
Inclusion Criteria
  • Previously impregnated their partner without the use of assisted reproduce technology, or previously had a normal sperm analysis exam by WHO 2010 criteria.
  • 1-2 months following the second dose of BNT162b2 COVID-19 Vaccine.
Exclusion Criteria
  • Known abnormal semen parameters.
  • Age above 45.

Study & Design

Study Type
OBSERVATIONAL
Study Design
Not specified
Arm && Interventions
GroupInterventionDescription
Vaccinated MenSperm collectionFertile men who were vaccinated with the BNT162b2 COVID-19 Vaccine
Primary Outcome Measures
NameTimeMethod
Semen PH1-2 months after the second COVID-19 vaccine

Semen PH

Sperm Concentration (10^6/ml)1-2 months after the second COVID-19 vaccine

Semen concentration (10\^6/ml)

Semen Volume (ml)1-2 months after the second COVID-19 vaccine

Semen Volume (ml)

Sperm Motility (%)1-2 months after the second COVID-19 vaccine

Sperm percentage with proper forward progression

White Blood Cells (10^6/ml) in Semen1-2 months after the second COVID-19 vaccine

Amount of white blood cells (10\^6/ml) in semen

Semen Viscosity (normal/abnormal)1-2 months after the second COVID-19 vaccine

Macroscopic appearance - Semen viscosity appearance

Sperm Morphology (%)1-2 months after the second COVID-19 vaccine

Sperm morphology (%)

Sperm Vitality (%)1-2 months after the second COVID-19 vaccine

Sperm vitality (%)

Semen Liquefaction (normal/abnormal)1-2 months after the second COVID-19 vaccine

Macroscopic appearance - Semen liquefaction appearance

Semen Color1-2 months after the second COVID-19 vaccine

Macroscopic appearance - Semen color appearance

Secondary Outcome Measures
NameTimeMethod

Trial Locations

Locations (1)

Sheba medical center

🇮🇱

Ramat Gan, Israel

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