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PredictEndTB Signature for Individualizing Treatment in Multidrug-Resistant Tuberculosis

Not Applicable
Terminated
Conditions
Mycobacterial Infection
Bacterial Infections
Tuberculosis, Pulmonary
Tuberculosis, Multidrug-Resistant
Interventions
Diagnostic Test: Individualised treatment duration based on RNA transcriptomic model
Registration Number
NCT04783727
Lead Sponsor
Research Center Borstel
Brief Summary

PredictEndTB signature is a non-inferiority, prospective, parallel-group open-label randomized controlled trial evaluating the efficacy of individualised antituberculous treatment durations that utilize the transcriptomic signature-based model compared to the standardised twenty months treatment in a cohort of multidrug-resistant tuberculosis patients.

Detailed Description

This study is a non-inferiority, prospective, parallel-group open-label randomized controlled trial. Three hundred forty-two HIV-negative patients diagnosed with pulmonary tuberculosis (TB) and starting a new anti-multidrug-resistant tuberculosis (MDR-TB) treatment cycle will be included in the study. Two randomized arms of 171 patients each will be recruited over the two-year period, each patient will be followed-up over the entire course of anti-TB treatment and one year after the end of therapy. Regular study visits will include physical examination, collection of sputum, blood and urine and filling in the study questionnaire. On the collected specimens standard bacteriological and blood tests, as well as extended immunological analysis, will be performed. In the experimental group, an RNA transcriptomic analysis using RNA-Seq technology will also be performed.

In the control arm, the patients will receive a standardised World Health Organization recommended 20 months treatment while in the experimental arm the treatment duration will be guided by the transcriptomic signature-based model.

Treatment outcomes and level of TB relapse and survival within the follow-up period will be compared between the experimental and control arms. The efficacy of biomarker-guided treatment therapy will be assessed by a comparison of the proportions of favourable study outcome between two arms.

Recruitment & Eligibility

Status
TERMINATED
Sex
All
Target Recruitment
4
Inclusion Criteria
  • Patient starting an MDR-TB treatment or within the first 4 weeks after treatment initiation and before culture conversion.
  • Rifampicin resistant M. tuberculosis detected in sputum using a nucleic acid amplification test.
  • New case of TB or re-treatment.
  • Can give informed consent at the point of recruitment.
  • Contactable (residing in the area covered by participating TB centres and possessing a landline or a mobile phone).
  • Willing to participate for the entire course of the treatment and extensive follow-up.
Exclusion Criteria
  • Age <18 years old.
  • Anti-MDR-TB therapy within 6 months prior to the start date of the current treatment cycle.
  • HIV infection.
  • Non-adherent patient with frequent interruptions.
  • Patient in custodianship or guardianship.
  • Late exclusion criterion: no positive cultures at inclusion and within the first 3 months of treatment.

Study & Design

Study Type
INTERVENTIONAL
Study Design
PARALLEL
Arm && Interventions
GroupInterventionDescription
Experimental armIndividualised treatment duration based on RNA transcriptomic modelThe individualised treatment durations defined by the RNA transcriptomic signature-based model
Primary Outcome Measures
NameTimeMethod
Proportion of patients with favourable study outcome 12 months after treatment endup to 36 months

The non-inferiority of the experimental arm compared to the control arm will be established if in the Per-Protocol population the difference in proportions of patients with a favourable study outcome between study arms is greater than the lower equivalence margin of 12%. This outcome measure is assessed after up to 24 months of treatment (usually 20 months) plus 12 months of follow-up after the end of treatment.

Secondary Outcome Measures
NameTimeMethod
Proportion of patients who had a treatment failureup to 24 months

This outcome measure will be used in the secondary efficacy analysis in the Intention-to-Treat population

Proportion of patients who died of any causeup to 36 months

This outcome measure will be used in the secondary efficacy analysis in the Intention-to-Treat population

Proportion of patients who was lost to follow-up during treatmentup to 24 months

This outcome measure will be used in the secondary efficacy analysis in the Intention-to-Treat population

Proportion of patients who died from TBup to 36 months

This outcome measure will be used in the secondary efficacy analysis in the Intention-to-Treat population

Trial Locations

Locations (5)

Research Center Borstel

🇩🇪

Borstel, Schleswig-Holstein, Germany

Marius Nasta Pulmonology Institute

🇷🇴

Bucharest, Romania

National Pirogov Memorial Medical University

🇺🇦

Vinnytsia, Ukraine

Kharkiv National Medical University

🇺🇦

Kharkiv, Ukraine

Phthisiopneumology Institute Chiril Draganiuc

🇲🇩

Chisinau, Moldova, Republic of

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