A Descriptive Study of Lymphocytic Phosphorylation of ERK1/2 in Patients With Parkinson's Disease With Dyskinesias and in Controls
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 发起方
- 入组人数
- 30
- 试验地点
- 1
- 主要终点
- ERK phosphorylation
研究概览
简要总结
Levodopa-induced dyskinesia severely limits the use of levodopa in Parkinson's disease and constitutes a debilitating complication of dopaminergic treatment in late stage. Among several neurobiological mechanisms identified so far, the investigators have established in experimental models the key role of D1 receptor hypersensitivity and a"Ras-ERK" signalling pathway. As the very same dopamine receptor machinery and the Ras-ERK pathway are present in blood lymphocytes, the investigators wish to test the hypothesis that the level of ERK phosphorylation in lymphocytes is a biomarker of levodopa-induced dyskinesia in Parkinson's Disease.
The study will be performed in dyskinetic levodopa-treated patients and non-Parkinson's Disease controls. Blood sampling "off" and "on" levodopa treatment (1 hour post-dose), as well as clinical data collection will be done during a scheduled pre-op work-up (deep brain stimulation). Subsequently, suspended lymphocytes from blood samples will be immunolabelled using an anti-pERK antibody and mean fluorescence intensity and percent of labelled lymphocytes will be assessed by flow cytometry. Additionally, plasma and urine samples will be collected "on" et "off" for dosage of dopamine. The motor effect of levodopa will be assessed through UPRSIII rating scale and eye movement (saccades) speed by non-invasive oculometric recordings.
研究设计
- 研究类型
- Observational
- 观察模型
- Case Control
- 时间视角
- Cross Sectional
入排标准
- 年龄范围
- 18 Years 至 85 Years（Adult, Older Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •Consecutive eligible PD in- and outpatients selected at the university hospital of Bordeaux.
- •Non-demented patients (DSM IV) who are able to give their informed consent and who are affiliated to the social security.
- •Controls: Subjects without known neurological disorder, non-demented, able to give their informed consent and affiliated to the social security.
排除标准
- •Patients: Atypical or secondary parkinson disease.
- •Previous or current cancer or malignant haemopathy.
- •Known auto-immune disease.
- •Anti-neoplastic or immuno-modulator treatment (particularly corticosteroids). Immuno-deficient subjects.
- •Acute viral infection (within 2 weeks after resolving). Statin drug intake. Demented subject (DSMIV).
- •Controls: Same criteria as above plus any neurological disease.
结局指标
主要结局
ERK phosphorylation
时间窗: Day 1
Distribution of variables and difference in the state of ERK phosphorylation in two contrasted groups : the dyskinetic levodopa-treated PD group and control group.
次要结局
- measure derivatives of morphine(Day 1)
- plasma and urinary dopamine in "on" and "off" state(Day 1)