Activation of Plasma miRNA and Proteins as a Result of Acute Experimental Pain
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 发起方
- 入组人数
- 20
- 试验地点
- 2
- 主要终点
- Protein expression over time
研究概览
简要总结
In this experiment, the investigators would like to test the following:
- Does acute pain induced by hypertonic injection cause changes in plasma miRNA and protein expression ?
- Can these canges correlate with the development of local hyperalgesia?
- How does the plasma miRNA expression change over a duration of 24 hours post pain induction ?
- Which mRNA targets are potentially affected by acute pain?
研究设计
- 研究类型
- Interventional
- 分配方式
- Randomized
- 干预模型
- Parallel
- 主要目的
- Basic Science
- 盲法
- Single (Participant)
入排标准
- 年龄范围
- 18 Years 至 80 Years（Adult, Older Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •Healthy men and women in the age 18-80 years
- •Speak and understand English
排除标准
- •Acute and chronic pain
- •Pregnancy or breastfeeding
- •Drug addiction defined as the use of cannabis, opioids or other drugs
- •Present or previous history of neurological, dermatological, immunological, musculoskeletal, cardiac disorder or mental illnesses that may affect the results (e.g. Neuropathy, muscular pain in the upper extremities, etc.)
- •Lack of ability to cooperate
- •Current use of medications that may affect the trial, such as antipsychotics and pain killers as well as systemic or topical steroids and anti-inflammatory drugs.
- •Skin diseases
- •Consumption of alcohol or painkillers 24 hours before the study days and between these
- •Participation in other trials within 1 week of study entry (4 weeks in the case of pharmaceutical trials)
研究组 & 干预措施
Hypertonic saline
Each participant will be given i.d. in 0.1 mL volumes of a hypertonic saline solution. The subject will be blinded to the hypertonic/isotonic administration.
干预措施: hypertonic saline (Other)
Hypertonic saline
Each participant will be given i.d. in 0.1 mL volumes of a hypertonic saline solution. The subject will be blinded to the hypertonic/isotonic administration.
干预措施: Blood sampling (Procedure)
Isotonic Saline
Each participant will be given i.d. in 0.1 mL volumes of a isotonic saline solution. The subject will be blinded to the hypertonic/isotonic administration.
干预措施: Saline injection (Other)
Isotonic Saline
Each participant will be given i.d. in 0.1 mL volumes of a isotonic saline solution. The subject will be blinded to the hypertonic/isotonic administration.
干预措施: Blood sampling (Procedure)
结局指标
主要结局
Protein expression over time
时间窗: 24 hours post-injection
Plasma protein evaluation
miRNA expression over time
时间窗: 24 hours post-injection
Plasma miRNA evaluation
miRNA expression over time
时间窗: Before injection
Plasma miRNA evaluation
miRNA expression over time
时间窗: 30 minutes post-injection
Plasma miRNA evaluation
miRNA expression over time
时间窗: 3 hours post-injection
Plasma miRNA evaluation
Protein expression over time
时间窗: Before injection
Plasma protein evaluation
Protein expression over time
时间窗: 30 minutes post-injection
Plasma protein evaluation
Protein expression over time
时间窗: 3 hours post-injection
Plasma protein evaluation
次要结局
- Assessment of pain: visual analog scale(20 minutes)
研究者
Silvia Lo Vecchio
Assistant Professor
Aalborg University