Effects of Dietary Nutrients on Liver and Adipose Tissue Metabolism

Not Applicable

• Conditions: Nutrient; Excess; NAFLD
• Interventions: Other: Overconsumption of specific dietary nutrient

• Registration Number: NCT03090347
• Lead Sponsor: University of Oxford

Brief Summary

In health,adipose tissue stores fat from the diet. If the fat tissue stops storing dietary fat then this increases the chance of it being stored in the liver, which is related to increased risk of heart disease and type 2 diabetes. It has been suggested that having a diet high in sugars or certain fats, may cause the fat tissue and liver to change how they function; we would like to investigate this in detail.

Detailed Description

This research aims to investigate how the overconsumption of specific macronutrients may effect adipose tissue and liver metabolism and function. Liver and heart fat content and adipose tissue and liver metabolism fasting and postprandial lipid metabolism will be assessed before and after the specific dietary intervention.

Basic Information
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Recruitment & Eligibility
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Study & Design
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Trial Locations

Study Timeline

• Study Start: 2017-03-01
• Study First Submitted: 2017-03-20
• Study First Submitted QC: 2017-03-20
• Study First Posted: 2017-03-24
• Status Verified: 2022-04
• Last Update Submitted: 2022-06-06
• Last Update Posted: 2022-06-07
• Primary Completion: 2023-03-31
• Study Completion: 2023-03-31

Recruitment & Eligibility

• Status: UNKNOWN
• Sex: All
• Target Recruitment: 100

Inclusion Criteria

• Participant is willing and able to give informed consent for participation in the study.
• Male or Female, aged ≥18 or ≤65 years.
• Body Mass Index ≥19 ≤35 kg/m2
• No medical condition or relevant drug therapy known to affect liver or adipose tissue metabolism.
• Weight stable for the previous 3 months

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Exclusion Criteria

• Patient is unwilling or unable to give informed consent for participation in the study
• A blood haemoglobin <135mg/dL for men and <120mg/dL for women
• Donated (or lost) ≥250 ml of blood in the previous two months.
• On a weight loss diet or have decreased their body weight by >5% in the previous 3 months.
• Have increased their body weight by >5% in the previous 3 months.
• Any metabolic condition or relevant drug therapy
• Current smoker
• History of alcoholism or a greater than recommended alcohol intake (>30 g of alcohol daily for men and >20 g of alcohol daily for women)
• Haemorrhagic disorders
• Anticoagulant treatment
• History of albumin allergy
• Pregnant or nursing mothers
• Women who are taking any contraceptive agent or device including oral contraceptives, hormone replacement therapy (HRT) or who have used these within the last 12 months
• History of severe claustrophobia
• Presence of metallic implants, pacemaker, or are unwilling to remove any piercings
• History of an eating disorder or any other psychological condition that may affect the participants ability to adhere to study intervention/experimental diets.

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Study & Design

• Study Type: INTERVENTIONAL
• Study Design: SINGLE_GROUP

Arm && Interventions

Group	Intervention	Description
High-sugar diet	Overconsumption of specific dietary nutrient	Participants will be asked to consume a relatively low-fat, high-carbohydrate eucaloric diet enriched in free-sugars (20% total energy).

Primary Outcome Measures

Name	Time	Method
Liver de novo lipogenesis	Within 21 days after starting the intervention diet	Investigators will utilise stable isotope tracer methodology to measure the change in the contribution of newly synthesised fatty acids to very low density lipoprotein triglyceride

Secondary Outcome Measures

Name	Time	Method
Liver fat accumulation	Within 21 days after starting the intervention diet	Investigators will measure the change in the amount of fat within the liver using MRI/S
Adipose tissue metabolism	Within 21 days after starting the intervention diet	Investigators will measure the change in the expression of key lipogenic genes in subcutaneous adipose tissue

Trial Locations

Locations (1)

Oxford Centre for Diabetes, Endocrinology and Metabolism; 🇬🇧 Oxford, United Kingdom