The Impact of Short-term Forearm Immobilization on Forearm Muscle Glucose and Amino Acid Metabolism of Volunteers With or Without Type 2 Diabetes
试验速览
- 阶段
- 不适用
- 状态
- 招募中
- 入组人数
- 26
- 试验地点
- 2
- 主要终点
- Change in forearm muscle glucose uptake
研究概览
简要总结
The aim of the present study is to assess the impact of short-term forearm immobilization on forearm muscle glucose uptake and amino acid net balance and kinetics in individuals with T2D compared with a control group with normoglycaemia.
详细描述
Participants with and without T2D will undergo 2 days of forearm immobilization as a model of local physical inactivity. Before, throughout, and after immobilization arteriovenous forearm balance measurements will be combined with a stable isotope tracer infusion to measure muscle glucose uptake and amino acid balance, uptake, and efflux.
研究设计
- 研究类型
- Interventional
- 分配方式
- Non Randomized
- 干预模型
- Parallel
- 主要目的
- Basic Science
- 盲法
- None
入排标准
- 年龄范围
- 18 Years 至 80 Years（Adult, Older Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •Males and females with or without diagnosed type 2 diabetes
- •Use lifestyle changes, metformin, gliclazide, or a combination thereof as oral glucose-lowering treatments for T2D
- •Aged 18-80 years at the time of signing informed consent
- •18.5 ≥ BMI ≤ 35 kg·m2
排除标准
- •Type 1 or a genetic form of diabetes
- •Any diagnosed cardiovascular (heart) disease or high blood pressure (≥160 mmHg systolic and/or ≥100 mmHg diastolic)
- •Chronic use of any prescribed or over-the-counter pharmaceuticals (excluding oral contraceptives and contraceptive devices) that interact with muscle substrate metabolism (e.g. selective serotonin reuptake inhibitors)
- •Consumption of a low-carbohydrate diet
- •Smoking or chewing tobacco
- •Known anaemia
- •Regular use of dietary protein and/or amino acid supplements (>3 times per week)
- •Currently involved in a structured progressive resistance training program (>3 times per week)
- •A personal or family history of thrombosis (clots)
- •Any previous motor disorders or inborn errors in muscle and/or lipid metabolism
- •History of kidney disease
- •History of liver disease
- •Pregnant or breastfeeding
- •History of any drug or alcohol abuse in the past two years
- •Claustrophobia
- •Unable to give consent
研究组 & 干预措施
Participants with Type 2 Diabetes
Two days of standardized meal consumption
干预措施: Forearm immobilization (Behavioral)
Participants without Type 2 Diabetes
Two days of standardized meal consumption
干预措施: Forearm immobilization (Behavioral)
结局指标
主要结局
Change in forearm muscle glucose uptake
时间窗: From 0 to 48 hours
The change in postprandial muscle insulin sensitivity, measured as forearm glucose uptake, from pre-immobilization to post-immobilization
次要结局
- Temporal change in forearm glucose uptake(Prior to and for 3.5 hours following every meal consumption during the 48 hours forearm immobilization period)
- Change in forearm muscle amino acid kinetics(Baseline and at regular intervals for 3.5 hours following the first and last meal consumption during the test period (0 to 48 hours))
- Change in whole-body substrate oxidation(Measurements will be performed at baseline, and at postprandial intervals (at 1 hour, 2 hours, 3 hours) during a 0 to 48-hour test period.)
- Change in forearm muscle amino acid kinetics(Baseline and at regular intervals for 3.5 hours following the first and last meal consumption during the test period (0 to 48 hours))
- Change in whole-body substrate oxidation(Measurements will be performed at baseline, and at postprandial intervals (at 1 hour, 2 hours, 3 hours) during a 0 to 48-hour test period.)
- Temporal change in forearm glucose uptake(Prior to and for 3.5 hours following every meal consumption during the 48 hours forearm immobilization period)
研究者
Marlou Dirks
Assistant Professor
Wageningen University