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Haploidentical Hematopoietic Cell Transplantation for Children With Hematologic Malignancies and Myelodysplasia

Phase 1
Active, not recruiting
Conditions
Myelodysplasia
Hematologic Malignancy
Interventions
Device: CliniMACS® TCRαβ/CD19 Combined Depletion System
Registration Number
NCT03431090
Lead Sponsor
Nationwide Children's Hospital
Brief Summary

This is a Phase I/II study designed to evaluate the kinetics of hematopoietic reconstitution and the incidence of acute chronic GVHD after partially matched related donor hematopoietic cell transplantation using an αβTCR/CD19+ cell depleted graft.

Detailed Description

Less than 30% of patients undergoing hematopoietic cell transplantation (HCT) will have an HLA-matched sibling donor. There is a high likelihood of being unable to identify a perfect HLA matched unrelated donor, and the time to procure the marrow if such a donor is available is generally \>3 months. An emerging body of literature suggests that related haploidentical HCT with innovative graft engineering may provide equal, or possibly superior, outcomes to conventional unrelated donors. This protocol is designed to test the hypothesis that HCT using an αβT cell / CD19+ B cell depleted graft from partially matched related donors will result in rapid, durable hematopoietic engraftment and rapid immune reconstitution with an acceptably low risk of severe acute and chronic GVHD.

Recruitment & Eligibility

Status
ACTIVE_NOT_RECRUITING
Sex
All
Target Recruitment
20
Inclusion Criteria
  • Patient lacks an HLA matched sibling donor.
  • Meets criteria nonhematopoietic organ function according to NCH BMT SOP09.
  • If subjects have received a first HCT, they must be eligible for a second HCT if their disease has recurred.
  • High resolution HLA and KIR typing
  • The subject cannot have an active untreated infection. Viremia by PCR analysis is not considered an active infection but may require immediate viral prophylaxis. Patients with possible fungal infections must have had at least 2 weeks of appropriate anti- fungal therapy and be asymptomatic.
  • Negative pregnancy test for females ≥11 years of age or post- menarche.
  • Sexually active males and females of childbearing potential must agree to use a form of contraception considered effective and medically acceptable by the Investigator. (Non-childbearing potential defined as pre-menarche, greater than one year post-menopausal or surgically sterilized).
  • Subjects must be ≤30 years at the time of consent.
  • Signed consent by parent/guardian and assent if appropriate for subjects < 18 years of age. Signed consent by patient/subject if ≥18 years of age.
Exclusion Criteria
  • Patient does not have a suitable donor who is willing and able (meets donor criteria).
  • Patient has donor-specific anti-HLA antibodies at the time of enrollment
  • Patient reports a history of allergic reactions to murine protein

Donor Eligibility:

  • The donor must be ≥18 years of age at the time of the informed consent conference.
  • The donor must be a related donor
  • The donor will be evaluated according to the current NCH BMT SOP 04 and must meet all criteria.
  • The donor must be able and willing to undergo G-CSF mobilization and stem cell apheresis.
  • The patient does not have donor specific anti-HLA antibodies

Study & Design

Study Type
INTERVENTIONAL
Study Design
SINGLE_GROUP
Arm && Interventions
GroupInterventionDescription
CliniMACS IsolationCliniMACS® TCRαβ/CD19 Combined Depletion SystemThe mobilized peripheral blood cell collection (apheresis product) will be processed using a Miltenyi CliniMACS device according to the manufacturing instructions. The processing will deplete the αβTCR+ cells and CD19+ cells from the apheresis product to formulate the graft.
Primary Outcome Measures
NameTimeMethod
Measure rates of immune reconstitution4 years

Patients undergoing partially matched related donor hematopoietic cell transplantation with an αβT cell / CD19+ B cell depleted graft should have a improved rate of immune reconstitution.

Measure incidence of chronic GVHD4 years

Patients undergoing partially matched related donor hematopoietic cell transplantation with an αβT cell / CD19+ B cell depleted graft should have a lower incidence of acute GVHD.

Measure rates of neutrophil and platelet engraftment4 years

Patients undergoing partially matched related donor hematopoietic cell transplantation with an αβT cell / CD19+ B cell depleted graft should have an improved rate of engraftment.

Measure incidence of acute GVHD4 years

Patients undergoing partially matched related donor hematopoietic cell transplantation with an αβT cell / CD19+ B cell depleted graft should have a lower incidence of acute and chronic GVHD.

Measure rates of platelet engraftment4 years

Patients undergoing partially matched related donor hematopoietic cell transplantation with an αβT cell / CD19+ B cell depleted graft should have an improved rate of engraftment.

Secondary Outcome Measures
NameTimeMethod
Measure overall survival4 years

Patients undergoing partially matched related donor hematopoietic cell transplantation with an αβT cell / CD19+ B cell depleted graft overall survival and relapse rate/disease free survival rates will be evaluated as compared to other treatment methodologies.

Measure relapse rate4 years

Patients undergoing partially matched related donor hematopoietic cell transplantation with an αβT cell / CD19+ B cell depleted graft overall survival

Measure disease free survival4 years

Patients undergoing partially matched related donor hematopoietic cell transplantation with an αβT cell / CD19+ B cell depleted graft overall survival

Define nonhematopoietic regimen related toxicities4 years

Patients undergoing partially matched related donor hematopoietic cell transplantation with an αβT cell / CD19+ B cell depleted graft will be evaluated for nonhematopoietic regimen related toxicities.

Trial Locations

Locations (1)

Nationwide Children's Hospital

🇺🇸

Columbus, Ohio, United States

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