New Electrophoretic Approaches in Studies of Obesity and Diabetes
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 发起方
- 入组人数
- 10
- 试验地点
- 1
- 主要终点
- Change in adipose tissue lipolysis in response to exercise
研究概览
简要总结
The aim of this study is to investigate whether metformin has inhibitory effect on exercise-induced lipolysis in subcutaneous adipose tissue in healthy lean man. Ten healthy lean mean will follow protocols consisting of 60 minutes of exercise on bicycle ergometer combined with a) orally administered metformin and b) locally administered metformin into adipose tissue and c) without metformin treatment. Microdialysis is used for assessment of metabolites in situ in subcutaneous adipose tissue.
详细描述
Metformin and physical activity are often recommended as the first therapeutic agents to improve glycemic control for prediabetes and type 2 diabetes (ADA). It has been reported that metformin decrease plasma concentration of non-esterified fatty acids in patients with type 2 diabetes which might be associated with the inhibition of lipolysis in adipose tissue. However, the inhibition of lipolysis during physical exercise, would not be probably beneficial. Thus, the effect of metformin on lipolysis during exercise is investigated in this protocol. As the lipolysis regulation is often impaired in obese and diabetic patients, the healthy lean men were chosen for this pilot study. Glycerol will be analyzed as a measure of lipolytic rate in adipose tissue and in plasma. Lactate levels will be observed. Our findings should provide a new background about the combination of physical activity and metformin medication.
研究设计
- 研究类型
- Interventional
- 分配方式
- Randomized
- 干预模型
- Crossover
- 主要目的
- Basic Science
- 盲法
- None
入排标准
- 年龄范围
- 25 Years 至 45 Years（Adult）
- 性别
- Male
- 接受健康志愿者
- 是
入选标准
- •healthy lean men, non obese, BMI 20-28 kg/m2
排除标准
- •weight change within the 3 months before the study,
- •hypertension,
- •impaired fasting glucose
- •hyperlipidemia
- •drug or alcohol abuse
研究组 & 干预措施
Metformin oral +exercise
Metformin, one dose 2000mg, 2.5h before the start of 60 min physical exercise
干预措施: Metformin Oral (Drug)
Metformin oral +exercise
Metformin, one dose 2000mg, 2.5h before the start of 60 min physical exercise
干预措施: Exercise (Other)
Metformin local in AT +exercise
Metformin perfused into microdialysis probe inserted in adipose tissue, 0.3ug/min 1.5h before the exercise and during 60min physical exercise
干预措施: Metformin local in AT (Drug)
Metformin local in AT +exercise
Metformin perfused into microdialysis probe inserted in adipose tissue, 0.3ug/min 1.5h before the exercise and during 60min physical exercise
干预措施: Exercise (Other)
No metformin + exercise
60min physical exercise without pretreatment of metformin
干预措施: Exercise (Other)
结局指标
主要结局
Change in adipose tissue lipolysis in response to exercise
时间窗: Minute -60, 15, 30, 45, 60 and 120 from the start of exercise
Glycerol concentration in dialysate outcoming from adipose tissue and in plasma - kinetic study
次要结局
- Change in lactate production in response to exercise(Minute -60, 15, 30, 45, 60 and 120 from the start of exercise)
研究者
Vladimir Stich
Professor, MUDr
Charles University, Czech Republic