Infrared Low-level Laser Therapy Before Intense Progressive Running Test of High-level Soccer Players
- Conditions
- Muscle; Fatigue, HeartSkeletal Muscle Recovery
- Interventions
- Device: Placebo PBMTDevice: Active PBMT
- Registration Number
- NCT03803956
- Lead Sponsor
- University of Nove de Julho
- Brief Summary
Photobiomodulation therapy (PBMT) has recently been used to alleviate postexercise muscle fatigue and enhance muscle recovery, demonstrating positive results. A previous study by the investigator's research group demonstrated the optimal dose and the optimal output power (100 mW) for an infrared wavelength (810 nm). However, the effects of optimized PBMT on performance and post-exercise recovery in high-level soccer players, to date have not been evaluated.
The present research project aims to evaluate the effects of PBMT (using low-level laser therapy) applied before a progressive running test on functional, muscle damage, inflammatory and oxidative stress markers in high-level soccer players.
- Detailed Description
To achieve the proposed objective it will perform a randomized, crossover triple-blinded, placebo-controlled trial, with high-level soccer players as volunteers.
Twenty-two volunteers will be randomly allocated to two intervention groups: active or placebo PBMT before a progressive running test (ergospirometry test).
The blood samples for biochemical analysis will be obtained before and after a progressive running test, and another outcomes (functional) will be obtained during the test.
The data will be collected by a blind assessor. The statistical analysis will follow the intention-to-treat principles and data on functional analysis will be analyzed using the paired and two-tailed t-student test. Data on biochemical analysis will be analyzed using two-way ANOVA, followed by Bonferroni post hoc test.
The investigators will analyze: functional aspects (rates of oxygen uptake -VO2max (absolute and relative), aerobic and anaerobic threshold and time until exhaustion), muscle damage (creatine-kinase -CK and lactate dehydrogenase -LDH), inflammatory markers (IL-1β, IL-6 and TNF-α) and oxidative stress markers (Thiobarbituric acid -TBARS, catalase - CAT, superoxidedismutase - SOD and carbonylated proteins).
Recruitment & Eligibility
- Status
- COMPLETED
- Sex
- Male
- Target Recruitment
- 22
- High-level soccer players;
- Age between 18 and 35 years;
- Male gender;
- Minimum of 80% participation in team practice sessions;
- Agreement to participate through signed statement of informed consent.
- History of musculoskeletal injury to hips or knees in previous 2 months;
- Use of pharmacological agents or nutritional supplements;
- Smokers and alcoholics;
- Occurrence of musculoskeletal injury during the trial;
- Any change in practice routine in relation to rest of the team during the trial.
Study & Design
- Study Type
- INTERVENTIONAL
- Study Design
- CROSSOVER
- Arm && Interventions
Group Intervention Description Placebo PBMT Placebo PBMT Application of placebo PBMT (Photobiomodulation Therapy) without any dose (0 Joule). Active PBMT Active PBMT Application of PBMT (Photobiomodulation Therapy) with a total dose of 850 Joules.
- Primary Outcome Measures
Name Time Method Rates of oxygen uptake (VO2max). Immediately after (1 minute) of ergospirometry test. Observation: the rates of oxygen uptake (relative and absolute) will be measured during the progressive running test. The rates of oxygen uptake will be measured by an ergospirometry test.
- Secondary Outcome Measures
Name Time Method Activity of creatine kinase - CK. The blood samples will be collected 5 minutes after the ergospirometry test (progressive running test). Muscle damage will be measured by blood samples.
Levels of interleukin 1 beta - IL-1b. The blood samples will be collected 5 minutes after the ergospirometry test (progressive running test). Inflammation will be measured by blood samples.
Levels of carbonylated proteins. The blood samples will be collected 5 minutes after the ergospirometry test (progressive running test). Oxidative stress will be measured by blood samples.
Activity of catalase - CAT. The blood samples will be collected 5 minutes after the ergospirometry test (progressive running test). Oxidative stress will be measured by blood samples.
Aerobic threshold. Immediately after (1 minute) of ergospirometry test. Observation: the aerobic threshold will be measured during the progressive runThe aerobic and anaerobic threshold will be measured during the progressive running test. The aerobic threshold will be measured by an ergospirometry test.
Activity of lactate dehydrogenase - LDH. The blood samples will be collected 5 minutes after the ergospirometry test (progressive running test). Muscle damage will be measured by blood samples.
Levels of Thiobarbituric acid - TBARS The blood samples will be collected 5 minutes after the ergospirometry test (progressive running test). Oxidative stress will be measured by blood samples.
Time until exhaustion. Immediately after (1 minute) of ergospirometry test (progressive running test). Observation: the time until exhaustion will be measured during the progressive running test. The time until exhaustion will be measured by the software of ergospirometry system.
Anaerobic threshold. Immediately after (1 minute) of ergospirometry test (progressive running test). Observation: the aerobic threshold will be measured during the progressive running test. The anaerobic threshold will be measured by an ergospirometry test.
Levels of interleukin 6 - IL-6. The blood samples will be collected 5 minutes after the ergospirometry test (progressive running test). Inflammation will be measured by blood samples.
Levels of tumor necrosis factor alpha - TNF-a. The blood samples will be collected 5 minutes after the ergospirometry test (progressive running test). Inflammation will be measured by blood samples.
Activity of superoxidedismutase - SOD. The blood samples will be collected 5 minutes after the ergospirometry test (progressive running test). Oxidative stress will be measured by blood samples.
Trial Locations
- Locations (1)
Laboratory of Phototherapy and Innovative Technologies in Health
🇧🇷São Paulo, Brazil