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Cold Plasma Therapy for Acceleration of Wound Healing in Diabetic Foot

Not Applicable
Active, not recruiting
Conditions
Diabetic Foot
Interventions
Device: Placebo
Device: Argon Plasma Jet
Registration Number
NCT04205942
Lead Sponsor
Ruhr University of Bochum
Brief Summary

Diabetic foot is a common complication of diabetes mellitus and requires specialized treatment. Wounds are characterized by persistent infection and chronic inflammatory processes, impeding well directed matrix remodelling and wound closure. Cold plasma applications have demonstrated beneficial effects on wound healing in several case reports. The investigator-initiated "Kaltplasma Wund (KPW)-Trial" was performed to prove beneficial effects of cold plasma in wound healing in a prospective, placebo-controlled, randomized bi-center study.

Detailed Description

Diabetic foot (DF) is a major complication in patients with diabetes leading to increased risk of hospitalization, lower limb amputation, and death, as well as a significant decrease in quality of life. During lifetime the risk for developing DF is assumed to be 25%; with 30% of DF resulting in lower limb amputation. If compared to general population people with diabetes have a 20-fold higher risk for amputation. Diabetes mellitus is the leading cause of lower limb amputation. Ulceration and impaired wound healing are commonly associated with common co-morbidities; the increased risk of infection amongst patients with diabetes is driving chronification and accounts for lack of wound healing.

Non-thermal atmospheric pressure plasma has been proposed as a tool for various biological and medical applications relying on its capacity to reduce bacterial load in the wound and to initiate wound healing. Biological plasma effects are largely dependent on plasma-generated reactive species in the gas phase, which subsequently diffuse or react with proteins and lipids in cells or tissues.

Thus, the objective of this placebo-controlled patient-blinded study was to show that application of cold plasma in addition to standard care treatment compared to placebo could accelerate wound healing in terms of more rapid and clinical meaningful wound surface regression. Wound closure progression and microbiological analysis were monitored time dependently to prove the effects. Patient's well-being and subjective perceptions were evaluated during treatment.

Recruitment & Eligibility

Status
ACTIVE_NOT_RECRUITING
Sex
All
Target Recruitment
65
Inclusion Criteria
  • Type 1 or Type 2 Diabetes mellitus
  • HbA1c ≤ 10%
  • at least one chronic wound persisting for at least three weeks without healing tendency following standard care wound therapy (Armstrong Wagner Grade Ib or IIb)
Exclusion Criteria
  • concomitant wound treatment with local vacuum therapy or maggot therapy
  • dialysis
  • use of topical active antibiotics,
  • concomitant treatment with platelet rich fibrin,
  • presence of critical limb ischemia defined as ankle brachial index below 0.5 or transcutaneous oxygen pressure below 15 mmHg.
  • participation in another clinical trial
  • women of child bearing potential without effective contraception or active breastfeeding

Study & Design

Study Type
INTERVENTIONAL
Study Design
PARALLEL
Arm && Interventions
GroupInterventionDescription
PlaceboPlaceboSham-treatment with Argon Plasma Jet, Plasma producing electric field switched off - no Plasma is produced, just argon gas as effluent, 8 times within 14 days
PlasmaArgon Plasma Jetregular treatment with Argon Plasma Jet according to manufacturer's instructions, 8 times within 14 days
Primary Outcome Measures
NameTimeMethod
change in wound surface areatreatment period of max 15 days

change in wound surface area within 14 days treatment

change in signs of clinical infectiontreatment period of max 15 days

change in clinical signs of infections as judged by the investigator

change in microbial loadtreatment period of max 15 days

change in microbial count, being evaluated by microbial culture

Secondary Outcome Measures
NameTimeMethod
time to significant wound surface area changetreatment period of max 15 days

time to 10% reduction of wound surface compared to treatment start

total wound surface change during treatmenttreatment period of max 15 days

total wound surface change compared to treatment start

treatment related side effects - formation of keloidstreatment period of max 15 days

question to evaluate potential side effects of the therapy: formation of keloids will be answered in a "yes" or "no" fashion and reported

treatment related side effects - presence of skin irritationtreatment period of max 15 days

question to evaluate potential side effects of the therapy: presence of skin irritation will be answered in a "yes" or "no" fashion and reported

changes in Quality of life (EQ5D questionnaire)treatment period of max 15 days

Evaluation of life quality during (EQ5D questionnaire) treatment period via questionaire

changes in Quality of life (SF12 questionnaire)treatment period of max 15 days

Evaluation of life quality during (SF12 questionnaire) treatment period via questionaire

time to change in wound infectiontreatment period of max 15 days

change in wound infection over treatment

treatment related side effects - local bleedingtreatment period of max 15 days

question to evaluate potential side effects of the therapy: local bleeding will be answered in a "yes" or "no" fashion and reported

treatment related side effects - proliferative skin reactiontreatment period of max 15 days

question to evaluate potential side effects of the therapy: proliferative skin reaction will be answered in a "yes" or "no" fashion and reported

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