Association of Intestinal Microbiota and the Onset of Perianal Abscess Based on 16S RDNA Amplicon Sequencing

Completed

• Conditions: Perianal Abscess
• Interventions: Procedure: surgical operation

• Registration Number: NCT05862129
• Lead Sponsor: Xi'an Hospital of Traditional Chinese Medicine

Brief Summary

The goal of this observational study is tolearn the association of gut microflora with the onset of perianal abscesses. The main questions it aims to answer are:

Question 1: To understand the structure of rectal microbial community composition and its relationship with pathogenic bacteria in patients with perianal abscess.

Question 2: Understand the structure of rectal microbial community composition in healthy people.

Participants will be collected with rectal secretions and stool specimens.In addition, patients with perianal abscess should also collect pus samples.

Detailed Description

Not available

Study Timeline

• Study First Submitted: 2023-05-07
• Study First Submitted QC: 2023-05-07
• Study First Posted: 2023-05-17
• Study Start: 2023-06-01
• Primary Completion: 2024-06-30
• Study Completion: 2024-06-30
• Status Verified: 2024-12
• Last Update Submitted: 2025-01-05
• Last Update Posted: 2025-01-07

Recruitment & Eligibility

• Status: COMPLETED
• Sex: All
• Target Recruitment: 100

Inclusion Criteria

• Observation group: ① Age between 18-60 years, local resident of Xi'an; ② met the diagnostic criteria for perianal abscess and underwent surgical treatment in our hospital; ③ did not use antibiotics before treatment; ④ himself and his family members were willing to participate in this study and signed informed consent.

Control group: ① Age between 18-60 years, permanent residents in Xi'an; ② had no serious chronic disease, no abnormality in physical examination in the last 1 week; ③ did not use drugs that may affect intestinal flora in the last one month; ④ himself and his family members are willing to participate in the study and sign the informed consent.

Exclusion Criteria

• Failing to retain the specimens as required

Study & Design

• Study Type: OBSERVATIONAL
• Study Design: Not specified

Arm && Interventions

Group	Intervention	Description
observation group	surgical operation	This group will collect rectal secretions and stool samples and pus samples from patients with perianal abscess before treatment, and collect rectal secretions and stool samples from patients again 2 months after treatment according to the diagnosis and treatment guidelines.

Primary Outcome Measures

Name	Time	Method
Observation group: the structure and changes of rectal flora before and after treatment	Two months	Analysis of the intestinal microflora based on 16S rDNA amplicon sequencing
Control group: the structure and changes of rectal flora in healthy population	Two months	Analysis of the intestinal microflora based on 16S rDNA amplicon sequencing

Trial Locations

Locations (1)

Xi'an Hospital of Traditional Chinese Medicine; 🇨🇳 Xi'an City, Shaanxi, China