Impact of aging and fitness on ischaemic preconditioning in humans

Recruiting

• Conditions: cardiovascular disease; 10011082; 10003216

• Registration Number: NL-OMON36213
• Lead Sponsor: niversitair Medisch Centrum Sint Radboud

Brief Summary

Not available

Detailed Description

Not available

Study Timeline

• Last Update Posted: 29 April 2024

Recruitment & Eligibility

• Status: Recruiting
• Sex: Not specified
• Target Recruitment: 45

Inclusion Criteria

-Healthy young volunteers : age 18-30
-Healthy sedentary older volunteers: age >55 years + sedentary (<1 h exercise per week)
-Healthy trained older volunteers: age >55 years + trained (>5 h exercise per week)
-All subjects: written informed consent

Exclusion Criteria

-Smoking
-History of any cardiovascular disease
-Hypertension (in supine position: systole >140 mmHg, diastole >90 mmHg)
-Diabetes Mellitus (fasting glucose >7.0 mmol/L or random glucose >11.0 mmol/L)
-Hyperlipidaemia (fasting total cholesterol >6.5 mmol/L)
-Chronic use of medication known to interfere with the cardiovascular system
-Professional athletes

Study & Design

• Study Type: Observational non invasive
• Study Design: Not specified

Primary Outcome Measures

Name	Time	Method
<p>Change in endothelial function (measured with flow mediated dilation) after<br /><br>ischaemia reperfusion injury (induced by 20 minutes ischemia) with and without<br /><br>precedence of ischaemic preconditioning (by 3 cycles of 5-minutes of<br /><br>ischaemia). </p><br>

Secondary Outcome Measures

Name	Time	Method
<p>Activation of PBMCs by plasma as measured in an ex-vivo assay.</p><br>