MedPath

Pathogenesis of Adverse Drug Reactions

Completed
Conditions
Seizures
Interventions
Other: No intervention; Urine Collection
Registration Number
NCT00224952
Lead Sponsor
Children's Mercy Hospital Kansas City
Brief Summary

The purpose of the study is to examine the individual metabolic profiles of pediatric patients receiving carbamazepine or valproate therapy, in an attempt to determine identities of the reactive metabolites or, alternatively, the identities of those metabolites that serve as potential precursors to reactive species.

Detailed Description

Adverse drug reactions can be broadly defined as any undesirable response associated with therapeutic drug use. A simple and clinically useful classification is to divide adverse events into those that are dose-dependent and largely predictable from the known pharmacologic properties of the compound in question, and those that are dependent on characteristics unique to susceptible individuals, or idiosyncratic in nature.

The long term objective of this research is to characterize the mechanisms responsible for the pathogenesis of idiosyncratic hypersensitivity reactions in children, particularly those involving carbamazepine and other aromatic anticonvulsants.

The study is divided into two phases. Phase 1 of the study involves collecting urine from 50 patients taking CBZ therapeutically. Participants will be asked to provide a spot urine sample during routine health visits. The urine will be analyzed for the presence of CBZ and its metabolites. In Phase 2 of the study, urine will be collected from patients taking either CBZ or VPA therapeutically. If blood samples are drawn from these patients for medical purposes not related to this study the residual blood sample will be recovered before it is discarded for use in genotyping analysis. Participants will be asked to provide a urine sample covering one complete dosing interval of CBZ or VPA (preferably overnight). Patients will also be followed longitudinally, with urine collections at each clinic visit over at least a two year period.

Recruitment & Eligibility

Status
COMPLETED
Sex
All
Target Recruitment
274
Inclusion Criteria
  • Pediatric patients of both genders between 1 and 16 years of age receiving CBZ or VPA mono-therapy will be recruited for this study. Additionally, for those patients who are receiving drugs other than CBZ or VPA to control their seizures, if CBZ or VPA are subsequently added to their treatment regimen, then these patients will also be recruited for this study.
Exclusion Criteria
  • None

Study & Design

Study Type
OBSERVATIONAL
Study Design
Not specified
Arm && Interventions
GroupInterventionDescription
Patients receiving Carbamazepine or Valproic AcidNo intervention; Urine Collection-
Primary Outcome Measures
NameTimeMethod
Drug (Valproic Acid or Carbamazepine) Metabolite Profiles in Urineurine samples (overnight collections) collected longitudinally through study completion for time frame ranging from 2-5 years

1. To examine the individual metabolic profiles of pediatric patients receiving carbamazepine or valproate therapy, in an attempt to determine the identities of the reactive metabolites or, alternatively, the identities of those metabolites that serve as potential precursors to reactive species (e.g., through conjugation with detoxifying compounds such as glutathione).

Secondary Outcome Measures
NameTimeMethod
Age-related Changes in Bioactivationurine samples (overnight collections) collected longitudinally through study completion for time frame ranging from 2-5 years

2. To determine if age-related differences exist regarding the ability of pediatric patients to bioactivate carbamazepine or valproate to reactive metabolites. Data provided below reflect the slope of the least squares regression.

Trial Locations

Locations (3)

Children's Mercy Hospital

🇺🇸

Kansas City, Missouri, United States

Primary Children's Hospital, Pediatric Neurology

🇺🇸

Salt Lake City, Utah, United States

Kosair Children's Hospital

🇺🇸

Louisville, Kentucky, United States

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