Polymorphonuclear Cells' Sensitivity to Aggregatibacter Actinomycetemcomitans Bacteria in Patients With Aggressive Periodontitis

Not Applicable

• Conditions: Periodontal Disease
• Interventions: Other: blood extraction

• Registration Number: NCT02010307
• Lead Sponsor: Polak David

Brief Summary

aggressive periodontitis is an inflammatory disease which damage the teeth supporting structures mostly in young patients. and has genetic basis.

a specific bacteria: Aggregatibacter actinomycetemcomitans (Aa) is associated with this disease, and found to damage leukocytes by using a specific leukotoxin.

in the research the investigators are aiming to find molecular and immunological basis to aggressive periodontitis.

Detailed Description

Not available

Study Timeline

• Status Verified: 2013-12
• Study First Submitted: 2013-12-09
• Study First Submitted QC: 2013-12-09
• Study First Posted: 2013-12-12
• Last Update Submitted: 2013-12-13
• Last Update Posted: 2013-12-16
• Study Start: 2014-01
• Primary Completion: 2015-01
• Study Completion: 2015-10

Recruitment & Eligibility

• Status: UNKNOWN
• Sex: All
• Target Recruitment: 75

Inclusion Criteria

• patients in periodontal department
• healthy
• diagnosed with chronic/aggressive periodontal disease
• interested in participating the research

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Exclusion Criteria

• diagnosed with diabetes, heart disease, immunosuppression, thrombocytopenia, clotting enzyme deficiency.
• using alcohol, or drugs
• pregnancy

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Study & Design

• Study Type: INTERVENTIONAL
• Study Design: PARALLEL

Arm && Interventions

Group	Intervention	Description
aggressive periodontitis	blood extraction	25 patients with aggressive periodontitis blood extraction
chronic periodontitis	blood extraction	25 patients with chronic periodontitis blood extraction
healthy	blood extraction	25 healthy periodontal patients blood extraction

Primary Outcome Measures

Name	Time	Method
flow cytometry	12 hours after blood extraction	the investigators use flow cytometry technique in order to determine the influence of gingival bacteria on the leukocytes, after culturing the two together.

Secondary Outcome Measures

Name	Time	Method
microscopy observation	12 hours after blood extraction	the investigators use microscopy in order to determine the influence of gingival bacteria on the leukocytes, after culturing the two together

Trial Locations

Locations (1)

Hadassah Medical Organization, Jerusalem, Israel; 🇮🇱 Jerusalem, Israel