The Effect of In-vitro Myoinositol Supplementation of Human Sperm on the Outcome of Cryopreservation
- Conditions
- HumanSpermCryopreservationMyoinositol
- Interventions
- Biological: Myoinositol
- Registration Number
- NCT03153436
- Lead Sponsor
- Sohag University
- Brief Summary
The objective of this study is to evaluate the effect of in-vitro myoinositol supplementation of human sperm on the outcome of cryopreservation.
- Detailed Description
Sperm cryopreservation is considered the most valuable and used way to preserve male reproductive function. Unfortunately, osmotic effects of freezing and thawing during the cryopreservation process negatively affect sperm morphology and motility .
Myoinositol is the most biologically important form of inositol in nature. In male reproductive system, myoinositol appears to regulate sperm motility, capacitation and acrosomal reaction.
The objective of this study is to evaluate the effect of in-vitro myoinositol supplementation of human sperm on the outcome of cryopreservation.
Recruitment & Eligibility
- Status
- COMPLETED
- Sex
- Male
- Target Recruitment
- 41
- Infertile men with normal or abnormal semen analysis
- Azoospermic samples
Study & Design
- Study Type
- INTERVENTIONAL
- Study Design
- PARALLEL
- Arm && Interventions
Group Intervention Description Abnormal S.A group Myoinositol Infertile men with abnormal semen analysis. each sample is divided into 2 identical aliquots: one aliquot (Myo aliquot) is supplied with myoinositol, The other aliquot is left as it is (control aliquot). Normal S.A group Myoinositol Infertile men with normal semen analysis. each sample is divided into 2 identical aliquots: one aliquot (Myo aliquot) is supplied with myoinositol, The other aliquot is left as it is (control aliquot).
- Primary Outcome Measures
Name Time Method Cryo-survival rate 7 months Post-thawing cryo-survival rates of both aliquots ( myo and control) are compared in both groups (A and B).
- Secondary Outcome Measures
Name Time Method