Anti-CD19/CD22 4th Generation CAR-T Cells (4SCAR19/22) Followed by Interleukin-2 Treating Pediatric Relapsed and Refractory B Cell Malignancies
试验速览
- 阶段
- 1 期
- 状态
- 撤回
- 发起方
- 试验地点
- 1
- 主要终点
- Evaluate the frequency and severity of adverse events including, but not limited to, cytokine release syndrome (CRS)
研究概览
简要总结
Clinical studies of CD19 CAR-T cells in the treatment of blood and lymphatic system tumors have achieved unprecedented successes. Because of the heterogeneity of the tumor, patients often carry CD19-negative tumor cell clones that express alternative target antigens (such as CD22, CD20 and CD123). In order to effectively eradicate all tumor clones and prevent recurrence, alternative tumor antigens besides CD19 are considered for CAR-T cell targeting. In this tudy, autologous T cells are genetically modified with 4th generation anti-CD19 and anti-CD22 CARs (4SCAR19/22) using lentiviral vectors. For safety consideration, the 4SCAR is engineered with an inducible caspase 9 self-withdrawal genetic design that allows for rapid elimination of the infused CAR-T cells. Interleukin-2 has been shown to boost immune response against leukemia cells. The serum interleukin-6 level will be monitored and when it returns to normal range by day 28 after CAR-T cell infusion, patients will receive subcutaneous injection of interleukin-2, and evaluated for 24 months for safety, efficacy and persistence of CAR T cells.
详细描述
Objectives:
-
Primary Outcome Measures:
-
Evaluate the frequency and severity of adverse events, including, but not limited to, cytokine release syndrome (CRS) [ Time Frame: From date of dosing ( day 1 ) up to 50 weeks ]
-
Evaluate grade 3 and higher toxicity rate of patients (toxicity possibly attributed to 4SCAR19/22 T cells)
-
Evaluate the safety and effect of administration of 4SCAR19/22 T cells followed by interleukin-2 in treating pediatric patients with relapsed and refractory B cell malignancies.
-
Secondary:
-
Evaluate Overall Complete Remission Rate (ORR).
-
Evaluate overall response rate including complete remission (CR) and complete remission with incomplete blood count recovery (CRi) .
-
Evaluate duration of remission (DOR).
-
To evaluate the incidence and the treatment effect of cytokine release syndrome (CRS).
-
To determine the expansion and functional persistence of 4SCAR19/22 T cells in the peripheral blood of patients and the correlation with antitumor effects;
Design:
研究设计
- 研究类型
- Interventional
- 分配方式
- Na
- 干预模型
- Single Group
- 主要目的
- Treatment
- 盲法
- None
入排标准
- 年龄范围
- 1 Year 至 14 Years（Child）
- 性别
- All
- 接受健康志愿者
- 否
入选标准
- •Relapsed or refractory CD19+ B-cell lymphoma or leukemia.
- •Measurable disease.
- •Karnofsky/jansky score of 60% or greater.
- •≥1 years old and ≤14 years.
- •Fertile females/males.
- •Expected survival>12 weeks.
- •Histologically confirmed as CD19/20-positive ALL/NHL and who meet one of the following conditions:
- •Patients receive at least 2-4 prior combination chemotherapy regimens (not including single agent monoclonal antibody therapy) and fail to achieve CR.
- •Recurrent disease and not eligible for allogeneic stem cell transplantation, and stable disease after therapy but refused further treatment.
- •Disease recurrence after stem cell transplantation.
- •Diagnosis as lymphoma, and refuse conventional treatment such as chemotherapy, radiation, stem cell transplantation and monoclonal antibody therapy.
- •Creatinine < 2.5 mg/dl.
- •Alanine transaminase (ALT) <3x upper limit of normal (ULN), aspartate aminotransferase (AST) <3x ULN.
- •Bilirubin < 2.0 mg/dl.
- •Adequate venous access for apheresis, and no other contraindications for leukapheresis.
- •Take contraceptive measures before recruit to this trial.
- •Written voluntary informed consent is given.
排除标准
- •A history of mental illness and poorly controlled.
- •Patients with symptoms of central nervous system.
- •Suffering severe cardiovascular or respiratory disease.
- •Accompanied by other malignant tumor.
- •Known human immunodeficiency virus (HIV) infection.
- •Active and/or severe infection (e.g. tuberculosis, sepsis and opportunistic infections, active hepatitis B virus (HBV) or active hepatitis C virus (HCV) infection).
- •Other serious underlying medical conditions, which, in the Investigator's judgment, could impair the ability of the patient.
- •Taking immunosuppressive agents within 1 week due to organ transplantation or other disease which need long-lasting administration.
- •Patients that do not consent to tissue and blood sample collection and storage in a biobank.
- •Female study participants of reproductive potential must have a negative serum or urine pregnancy test performed 48 hours before infusion.
- •Pregnancy and nursing females.
研究组 & 干预措施
4SCAR19/22 T cells and interleukin-2
Patients with resistant or refractory B cell acute lymphoblastic leukemia (ALL) or non-hodgkin's lymphoma (NHL) will receive CAR-T cells at a total dose of 0.5-5x10^6/kg and regular subcutaneous injection of interleukin-2 every other day for 2 weeks and then rest for 2 weeks for up to 6 months after their serum interleukin-6 levels returned to normal range from day 28 after CAR-T cell infusion.
干预措施: 4SCAR19/22 T cells (Biological)
4SCAR19/22 T cells and interleukin-2
Patients with resistant or refractory B cell acute lymphoblastic leukemia (ALL) or non-hodgkin's lymphoma (NHL) will receive CAR-T cells at a total dose of 0.5-5x10^6/kg and regular subcutaneous injection of interleukin-2 every other day for 2 weeks and then rest for 2 weeks for up to 6 months after their serum interleukin-6 levels returned to normal range from day 28 after CAR-T cell infusion.
干预措施: Interleukin-2 (Drug)
结局指标
主要结局
Evaluate the frequency and severity of adverse events including, but not limited to, cytokine release syndrome (CRS)
时间窗: From date of dosing ( day 1 ) up to 50 weeks
b)Evaluate grade 3 and higher toxicity rate of patients (toxicity possibly attributed to 4SCAR19/22 T cells)
次要结局
未报告次要终点