Efficacy of Hypochlorous Acid as a Postoperative Wound Antiseptic

Not Applicable

Completed

• Conditions: Wound Healing
• Interventions: Drug: Hypochlorous acid

• Registration Number: NCT06760403
• Lead Sponsor: Ataturk University

Brief Summary

The aim was to examine the effectiveness of hypochlorous acid on postoperative wound healing in 24 systemically healthy gingivectomy participants aged 12-30. The participants were divided into 3 groups as hypochlorous acid group, chlorhexidine group and saline group and their wound healing was evaluated on the 1st, 3rd and 7th days.

Detailed Description

The aim of this study was to investigate the effectiveness of hypochlorous acid on postoperative wound healing in 24 participants aged 12-30 who underwent gingivectomy involving the 6 anterior teeth of the upper or lower jaw with chronic inflammatory gingival overgrowth. The participants were divided into 3 groups as hypochlorous acid group, chlorhexidine group and saline group and were called for control on the 1st, 3rd and 7th days to evaluate wound healing. Periodontal indexes, visual analog scale (VAS), H2O2, plaque staining agent test, and histological analyzes were used to evaluate wound healing. The data were analyzed statistically.

Study Timeline

• Study Start: 2023-05-02
• Primary Completion: 2023-10-02
• Study Completion: 2023-12-01
• Study First Submitted: 2024-12-24
• Status Verified: 2025-01
• Study First Submitted QC: 2025-01-03
• Last Update Submitted: 2025-01-03
• Study First Posted: 2025-01-06
• Last Update Posted: 2025-01-06

Recruitment & Eligibility

• Status: COMPLETED
• Sex: All
• Target Recruitment: 24

Inclusion Criteria

• Gingival overgrowth affecting at least six teeth symmetrically in the mandibular or maxillary anterior area
• No attachment and bone loss
• 7 days before the surgery, full-mouth plaque index (PI) < 25% and a full-mouth gingival bleeding index (GBI) < 25%.
• the keratinized tissues surrounding the teeth are at least 2 mm wide.

Exclusion Criteria

• Smoking or alcohol use
• Patients who have undergone systemic antimicrobial therapy and surgical procedures within the last 3 months
• Pregnancy or lactation
• Immunodeficiency or immunosuppressive drug therapy

Study & Design

• Study Type: INTERVENTIONAL
• Study Design: PARALLEL

Arm && Interventions

Group	Intervention	Description
Hypochlorous Group	Hypochlorous acid	In the hypochlorous acid group, 15 ml was used as a postoperative gargle, 2 times \* 7 days.
Chlorhexidine Group	Hypochlorous acid	In the clorhexidine group, 15 ml was used as a postoperative gargle, 2 times \* 7 days.
Saline Group	Hypochlorous acid	In the saline group, 15 ml was used as a postoperative gargle, 2 times \* 7 days.

Primary Outcome Measures

Name	Time	Method
Plaque index	Baseline, Day 7	The amount of plaque was scored.
Probing depth	Baseline, Day 7	The periodontal pocket depth was performed (mm).
Clinical attachment level	Baseline, Day 7	The clinical attachment level was measured (mm).
Gingival index	Baseline, Day 7	The gingival status was scored.
Epithelialization with plaque staining agent	Day 1, 3, and 7	The wound surface epithelialization was observed with plaque staining agent. The wound surface was stained with plaque staining dye and the dark colored area is calculated as mm2.
Histological analysis	Day 1, 3, and 7	Histological analysis evaluated epithelial keratinization, regeneration, and/or degeneration during the healing process and to obtain epithelial samples repeatedly. The number of keratinocytes was counted.
Bleeding on probing	Baseline, Day 7	The gingival status was scored.(%)
Epithelialization with H2O2	Day 3 and 7	The wound surface epithelialization was observed with H2O2. Scored as foaming present/absent after application with H2O2
postoperative pain with Visiual Analogue Scale (VAS)	Day 3 and 7	VAS scores were taken on the 3rd and 7th days without any intervention to the patient. Scored between 1-10 as 1 was no pain, 10 was pain.

Trial Locations

Locations (1)

Atatürk University Faculty of Dentistry; 🇹🇷 Erzurum, Turkey