Determining the Extra-pancreatic Effects of Incretin Hormones During Euglycemic and Hyperglycemic Pancreatic Clamps
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 入组人数
- 20
- 试验地点
- 1
- 主要终点
- Glucose metabolism
研究概览
简要总结
Incretin hormones (GLP-1 and GIP) released from the intestine in response to meal ingestion augment insulin secretion from the pancreas to help maintain glycemic control. Studies in vitro and in vivo have shown that these incretin hormones also have functional effects in other tissues independent of the insulin secretory response. Both GLP-1 and GIP stimulate insulin secretion in a glucose-dependent manner, however the glucose-dependency of their extra-pancreatic effects has not been examined in vivo. By using pancreatic clamp methodology during euglycemic and hyperglycemic conditions we will test the hypothesis that extra-pancreatic effects of GLP-1 and GIP are glucose-dependent.
研究设计
- 研究类型
- Interventional
- 分配方式
- Randomized
- 干预模型
- Crossover
- 主要目的
- Basic Science
- 盲法
- None
入排标准
- 年龄范围
- 18 Years 至 60 Years（Adult）
- 性别
- Male
- 接受健康志愿者
- 是
入选标准
- •age 18-60 years
- •BMI 18-30 kg/m2
- •Normal glycemic control (fasting glucose <5.6 mM)
排除标准
- •Evidence of chronic disease
- •Active weight loss (>2 kg in previous 6 months)
- •Treatment with drugs known to affect our outcome varaibles
研究组 & 干预措施
GLP-1
GLP-1 will be infused intravenously during the pancreatic clamp at 0.5 pmol/kg/min
干预措施: GLP-1 (Biological)
结局指标
主要结局
Glucose metabolism
时间窗: up to 4 hours
Pancreatic clamp will be performed including infusion of somatostatin and replacement of basal insulin, glucagon, and growth hormone. During pancreatic clamps, euglycemia (5 mM) will be maintained via exogenous glucose infusion for the first 2-hours, followed by hyperglycemia (+5 mM) for the final 2-hours. An infusion of the stable isotope \[U13C\]glucose will be performed to assess glucose kinetics. Expired air will be collected for the analysis of \[U13C\]glucose into 13CO2.
Lipid metabolism
时间窗: up to 4 hours
An infusion of the stable isotope \[D5\]glycerol will be performed to assess glycerol kinetics.
次要结局
- Endothelial function(Baseline, 2 hours, and 4 hours)
- Signaling(Baseline, 2 hours, and 4 hours)
研究者
Thomas Solomon
Senior Researcher
Rigshospitalet, Denmark