Transition from pro-inflammatory to reparative phase following acute myocardial infarction: Role of the innate immune system.
Recruiting
- Conditions
- I21Acute myocardial infarction
- Registration Number
- DRKS00033039
- Lead Sponsor
- niversitätsklinikum Freiburg Abteilung Kardiologie und Angiologie
- Brief Summary
Not available
- Detailed Description
Not available
Recruitment & Eligibility
- Status
- Recruiting
- Sex
- All
- Target Recruitment
- 100
Inclusion Criteria
ST-Segment elevation in ECG and interventional recanalisation with stenting.
Exclusion Criteria
None.
Study & Design
- Study Type
- observational
- Study Design
- Not specified
- Primary Outcome Measures
Name Time Method At indicated timepoints NLRP3 expression in neutrophils is absolutely quantified using digital droplet PCR as well as the intercellular levels of the NLRP3 protein determined using ELISA. The hypothesis being that 1) Elevated NLRP3 expression and transcription in neutrophils is characteristic of ischemia-reperfusion injury following acute myocardial infarction and 2) the timeline of alteration in neutrophil NLRP3 expression and transcription is indicative of the switch from the inflammatory phase to the reparative phase following myocardial infarction indicated by corresponding changes in plasma inflammatory markers measured using ELISA.<br><br>
- Secondary Outcome Measures
Name Time Method Within 24 hours of inclusion and after 30 days, the resulting myocardial damage is quantified using 3D speckle tracking echocardiography (STE) and the ejection fraction is determined. In addition, after 30 days, the size of the resulting infarction is precisely quantified using contrast-enhanced magnetic resonance imaging (CE-MRI).<br>The hypothesis is that the extent and temporal dynamics of neutrophil NLRP3 activation correlates with the extent of the inflammatory immune response after myocardial infarction and thereby significantly affects the extent of myocardial damage.