Consumption of fish oil and a Mediterranean influenced antioxidant diet on telomere length in older Australians: Adherence and feasibility from a pilot study

Not Applicable

Completed

• Conditions: At risk of cardiovascular disease; Cardiovascular - Other cardiovascular diseases

• Registration Number: ACTRN12613000875707
• Lead Sponsor: Flinders University

Brief Summary

Not available

Detailed Description

Not available

Study Timeline

• Results First Posted: 1900-01-01
• Study Start: 2013-08-06
• Last Update Posted: 13 January 2020

Recruitment & Eligibility

• Status: Completed
• Sex: All
• Target Recruitment: 30

Inclusion Criteria

(1) be aged greater than or equal to 60 years; and (2) have any one of the following conditions (indicating increased risk for CVD: i. diabetes; ii. diagnosed familial hypercholesterolaemia; iii. systolic blood pressure greater than or equal to 180 mmHg or diastolic blood pressure greater than or equal to 110 mmHg; iv. serum total cholesterol greater than 7.5 mmol/L or any two of the following: i. current smoker; ii. waist circumference (greater than or equal to 94 cm men; greater than or equal to 80 cm women); iii. BMI (greater than or equal to 25.0 kg/m^2); iv. family history of CVD; and 3) a low intake of fish (less than 1 serving per week) or self-report to consume nuts and olive oil in amounts lower than the proposed Mediterranean style diet; (4) willing to consume 9 fish oil capsules per day or follow a diet containing foods high in antioxidants; (5) able to provide written informed consent; and (6) able to attend clinic visits at Flinders Medical Centre.

Exclusion Criteria

(1) unwilling to comply with study protocol; (2) diagnosed cardiovascular disease or suffered a CV event; (3) currently on a weight loss diet or actively trying to lose weight; (4) allergies to fish/seafood or components of the Mediterranean diet (e.g. nuts); (5) intake of anti-inflammatory medications on a regular basis or if an acute intake, within 1 week of clinic screening visit; and (6) currently taking fish oil.

Study & Design

• Study Type: Interventional
• Study Design: Not specified

Primary Outcome Measures

Name	Time	Method
Adherence to the MAx diet through a validated Mediterranean diet score, self-reported daily diaries stating weekly nut consumption, and self-reported remaining olive oil.[Baseline, 6 weeks and 12 weeks];Adherence to fish oil capsules through fish oil diaries that recorded daily capsule consumption, adverse reactions, and reasons for missed doses.[Baseline and 12 weeks]

Secondary Outcome Measures

Name	Time	Method
Telomere length measured in peripheral blood mononuclear cells isolated from whole blood and analysed using the qPCR technique.[Baseline and 12 weeks.];Telomerase activity measured using the repeat amplification protocol (TRAPeze 'registered trademark' ELISA Telomerase Detection Kit)[Baseline and 12 weeks]