Does Notch Regulate Glioma Proliferation ?

Completed

• Conditions: Glioma

• Registration Number: NCT04809597
• Lead Sponsor: University Hospital, Montpellier

Brief Summary

The investigators showed in 2015 (Guichet et al) that the Notch1 pathway has an anti-proliferative role on glial brain tumors. In this project, the investigators investigated which genes downstream of Notch1 pathway activation produce the anti-proliferative effect. The investigators hypothesized that the SNAI2 and TAL1 genes act downstream of Notch1

Detailed Description

Main objectives:

To examine the expression of TAL1 and SNAI2 in protein extracts and glioma sections of different grades (glioblastomas and diffuse low-grade tumors).

To examine the effect of overexpression of Tal1 and Snai2 in high grade glioma cultures.

To show the expression of Tal1 and SNAI2 by tumor cells by FISH technique. Show that TAL1 and SNAI2 could be regulated by endothelial cells

Study Timeline

• Study Start: 2012-01-01
• Primary Completion: 2021-01-01
• Study Completion: 2021-01-20
• Status Verified: 2021-03
• Study First Submitted: 2021-03-17
• Study First Submitted QC: 2021-03-18
• Last Update Submitted: 2021-03-18
• Study First Posted: 2021-03-22
• Last Update Posted: 2021-03-22

Recruitment & Eligibility

• Status: COMPLETED
• Sex: All
• Target Recruitment: 10

Inclusion Criteria

Not provided

Exclusion Criteria

• Patient who reject the study protocol
• Radiotheray or chemotherapy treatment

Study & Design

• Study Type: OBSERVATIONAL
• Study Design: Not specified

Primary Outcome Measures

Name	Time	Method
Snai2 gene and protein expression	1 day	western blot for Tal1 and Snai2 in protein extracted from low grade and high gliomas
Effect of Notch1 activation on Tal1	1 day	highthroughput gene analysis (DNA arrays) with cells transduced with activated form of Notch1

Trial Locations

Locations (1)

Uhmontpellier; 🇫🇷 Montpellier, France