Study of the Activity and Role of Notch1 Signalling in Glioma
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 入组人数
- 10
- 试验地点
- 1
- 主要终点
- Snai2 gene and protein expression
研究概览
简要总结
The investigators showed in 2015 (Guichet et al) that the Notch1 pathway has an anti-proliferative role on glial brain tumors. In this project, the investigators investigated which genes downstream of Notch1 pathway activation produce the anti-proliferative effect. The investigators hypothesized that the SNAI2 and TAL1 genes act downstream of Notch1
详细描述
Main objectives:
To examine the expression of TAL1 and SNAI2 in protein extracts and glioma sections of different grades (glioblastomas and diffuse low-grade tumors).
To examine the effect of overexpression of Tal1 and Snai2 in high grade glioma cultures.
To show the expression of Tal1 and SNAI2 by tumor cells by FISH technique. Show that TAL1 and SNAI2 could be regulated by endothelial cells
研究设计
- 研究类型
- Observational
- 观察模型
- Case Only
- 时间视角
- Retrospective
入排标准
- 年龄范围
- 18 Years 至 —（Adult, Older Adult）
- 性别
- All
- 接受健康志愿者
- 否
入选标准
- 未提供
排除标准
- •Patient who reject the study protocol
- •Radiotheray or chemotherapy treatment
结局指标
主要结局
Snai2 gene and protein expression
时间窗: 1 day
western blot for Tal1 and Snai2 in protein extracted from low grade and high gliomas
Effect of Notch1 activation on Tal1
时间窗: 1 day
highthroughput gene analysis (DNA arrays) with cells transduced with activated form of Notch1
次要结局
未报告次要终点