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Belatacept Pilot Study in Lung Transplantation Immunosuppression in Lung Transplantation

Phase 2
Completed
Conditions
Antibody-mediated Rejection
Lung Transplant Rejection
Interventions
Registration Number
NCT03388008
Lead Sponsor
Washington University School of Medicine
Brief Summary

This is a pilot randomized controlled trial examining the feasibility of conducting a large scale randomized controlled trial of belatacept-based immunosuppression in lung transplantation. This pilot study will enroll 40 lung transplant recipients and randomize them to belatacept-based immunosuppression or standard of care. The primary endpoint of the study is the development of donor-specific HLA antibodies after transplantation. All study participants will be followed for a minimum of 12 months after transplantation.

Detailed Description

Lung transplantation is the ultimate treatment for patients with advanced lung disease. However, long-term outcomes remain disappointing and the median survival after transplantation is approximately 5.5 years. Beyond the first year after transplantation, chronic lung allograft dysfunction is the leading cause of death. The exact mechanisms that lead to chronic lung allograft dysfunction are unclear, but the development of donor-specific HLA antibodies is an independent risk factor. In fact, studies have consistently identified the development of donor-specific HLA antibodies as a significant and independent risk factor for chronic lung allograft dysfunction and mortality after transplantation.

Belatacept is a CTLA4-Ig fusion protein that binds CD80 and CD86 thereby blocking CD28 co-stimulatory signals. Belatacept has been extensively studied in kidney transplantation. In a long-term study, patients treated with Belatacept had better survival than those treated with Cyclosporine. Importantly, Belatacept-treated patients were significantly less likely to develop donor-specific HLA antibodies than Cyclosporine-treated patients. Nonetheless, Belatacept has not been formally evaluated after lung transplantation. The investigators hypothesize that Belatacept-based immunosuppression would result in a lower incidence of donor-specific HLA antibodies and that this would result in better chronic lung allograft dysfunction-free survival after transplantation. Before conducting a large scale randomized controlled trial to test this hypothesis, the investigators plan to conduct the current pilot randomized controlled trial to examine the feasibility of conducting the large scale randomized controlled trial.

The investigators plan to enroll and randomize 40 lung transplant recipients at 2 sites. All recipients will be treated with anti-thymocyte globulin for induction immunosuppression. Those randomized to standard of care immunosuppression will be treated with Tacrolimus, Mycophenolate mofetil, and prednisone. Those randomized to Belatacept-based immunosuppression will be treated with Belatacept, Tacrolimus, and prednisone for the first 89 days; on day 90, Mycophenolate mofetil will be substituted for Tacrolimus and patients will be continued on Belatacept, Mycophenolate mofetil, and prednisone for the remainder of year 1 after transplantation.

Patients in both groups will be monitored closely for episodes of acute cellular rejection, lymphocytic bronchiolitis, and antibody-mediated rejection with surveillance bronchoscopy and transbronchial lung biopsies on days 28, 84, 112, 168, 252, and 365 (± 7 days) as part of the sites' routine clinical protocols. In addition, patients will be monitored for the development of donor-specific HLA antibodies with routine blood tests on on days 10 (± 3 days), 28, 56, 84, 112, 168, 252, and 365 (± 7 days).

The primary endpoint of the study is a composite of the development of donor-specific HLA antibodies, re-transplantation, and death. Secondary endpoints include acute cellular rejection, lymphocytic bronchiolitis, antibody-mediated rejection, chronic lung allograft dysfunction, survival, cytomegalovirus infection, bacterial infection, community-acquired respiratory viral infection, chronic kidney disease stage 3, malignancy, hypertension, diabetes, and hypercholesterolemia.

Recruitment & Eligibility

Status
COMPLETED
Sex
All
Target Recruitment
27
Inclusion Criteria
  • Provided written informed consent for study participation
  • Underwent single or bilateral lung transplantation
  • Negative urine pregnancy test for women of child bearing potential and willingness to use highly-effective contraception
Exclusion Criteria
  • Requiring invasive mechanical ventilation immediately before transplantation
  • Requiring extracorporeal life support (ECLS) (i.e., ECMO) immediately before transplantation
  • Received treatment to deplete HLA antibodies before transplantation to improve the possibility of transplantation
  • Having DSA immediately before transplantation (i.e., positive virtual crossmatch)
  • Listed for multi-organ transplant (e.g., heart-lung, liver-lung, kidney-lung)
  • Pregnant or breast-feeding
  • Active infection with Hepatitis B or C virus
  • Active infection with human immunodeficiency virus (HIV)
  • Chronic infection with Burkholderia cepacia complex before transplantation
  • Epstein Barr Virus (EBV) seronegative status
  • Participation in another interventional clinical trial
  • Allograft dysfunction requiring ECMO support after transplantation
  • Delayed chest closure after transplantation
  • Severe coagulopathy and significant bleeding in the opinion of the PI
  • Any condition that in the opinion of the site PI introduces undue risk by participating in this study

Study & Design

Study Type
INTERVENTIONAL
Study Design
PARALLEL
Arm && Interventions
GroupInterventionDescription
Standard of careTacrolimusTacrolimus + Mycophenolate mofetil + prednisone from day 0 through day 365
Standard of careMycophenolate MofetilTacrolimus + Mycophenolate mofetil + prednisone from day 0 through day 365
Belatacept-based immunosuppressionBelataceptBelatacept + Tacrolimus + prednisone from day 0 through day 89, then Belatacept + Mycophenolate mofetil + prednisone from day 90 through day 365
Standard of careMethylprednisoloneTacrolimus + Mycophenolate mofetil + prednisone from day 0 through day 365
Standard of careATGTacrolimus + Mycophenolate mofetil + prednisone from day 0 through day 365
Standard of carePrednisoneTacrolimus + Mycophenolate mofetil + prednisone from day 0 through day 365
Belatacept-based immunosuppressionATGBelatacept + Tacrolimus + prednisone from day 0 through day 89, then Belatacept + Mycophenolate mofetil + prednisone from day 90 through day 365
Belatacept-based immunosuppressionMethylprednisoloneBelatacept + Tacrolimus + prednisone from day 0 through day 89, then Belatacept + Mycophenolate mofetil + prednisone from day 90 through day 365
Belatacept-based immunosuppressionMycophenolate MofetilBelatacept + Tacrolimus + prednisone from day 0 through day 89, then Belatacept + Mycophenolate mofetil + prednisone from day 90 through day 365
Belatacept-based immunosuppressionPrednisoneBelatacept + Tacrolimus + prednisone from day 0 through day 89, then Belatacept + Mycophenolate mofetil + prednisone from day 90 through day 365
Primary Outcome Measures
NameTimeMethod
Donor-specific HLA Antibodies, Re-transplantation, or Death365 days

The Outcome Measure is a composite primary endpoint of the development of donor-specific HLA antibodies, re-transplantation, or death. Testing for donor-specifc HLA antibodies was performed at study-specified time points using the single antigen bead assay at the study core lab. Donor-specific HLA antibodies were defined as reactivity with a mean fluorescence intensity (MFI) ≥ 2,000.

Secondary Outcome Measures
NameTimeMethod

Trial Locations

Locations (2)

Washington University School of Medicine

🇺🇸

Saint Louis, Missouri, United States

Houston Methodist Hospital

🇺🇸

Houston, Texas, United States

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