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Vitamin D and Pregnancy Outcome in PCOS Patients

Not Applicable
Completed
Conditions
in Vitro Fertilization
Polycystic Ovary Syndrome
Interventions
Other: Placebo
Registration Number
NCT04082650
Lead Sponsor
Women's Hospital School Of Medicine Zhejiang University
Brief Summary

This multicenter double-blinded placebo-controlled randomised trial aims to evaluate the effectiveness of vitamin D supplementation prior to IVF on the live birth rate in women with PCOS. Women with PCOS scheduled for IVF will be enrolled. Eligible participants will be randomised 1:1 to receive oral capsules of 4000IU vitamin D per day or placebo for around 12 weeks until the day of triggering.

Detailed Description

Polycystic ovary syndrome (PCOS) is one of the leading causes of female infertility, affecting around 5% of women with a child-bearing age in China. Vitamin D insufficiency is common in women with PCOS and is associated with lower live birth rates in these women. However, evidence regarding the effectiveness of vitamin D treatment in women with PCOS is inconclusive. This multicenter double-blinded placebo-controlled randomised trial aims to evaluate the effectiveness of vitamin D supplementation prior to IVF on the live birth rate in women with PCOS.

The investigators plan to enroll women with PCOS scheduled for IVF. Eligible participants will be randomised 1:1 to receive oral capsules of 4000IU vitamin D per day or placebo for around 12 weeks until the day of triggering. All IVF procedures will be carried out following a routine method in each center. Women will be followed up until six months after randomisation for those who fail to get pregnant after the completion of the first embryo transfer or delivery for those who get pregnant after the first embryo transfer. The primary outcome is live birth after the first embryo transfer. Primary analysis will be by intention-to-treat analysis. The investigators plan to recruit 860 women to demonstrate women treated with vitamin D have a higher live birth rate than those treated with placebo (48% versus 38%), accounting for 10% loss to follow-up with a significance level at 0.05 and a power of 80%.

Recruitment & Eligibility

Status
COMPLETED
Sex
Female
Target Recruitment
876
Inclusion Criteria

Not provided

Exclusion Criteria

Not provided

Study & Design

Study Type
INTERVENTIONAL
Study Design
PARALLEL
Arm && Interventions
GroupInterventionDescription
PlaceboPlaceboParticipants in the control group will be treated with equal amount of placebo tablets per day for the same duration.
Vitamin Dvitamin DParticipants in the intervention group will be treated with vitamin D 4000IU (800IU per pill, take five pills once each day) per day for around 12 weeks (till the triggering day).
Primary Outcome Measures
NameTimeMethod
Number of Participants with live birth1 day after delivery

The primary outcome is live birth after the first embryo transfer. Live birth is defined as the delivery of at least one baby after 24 weeks of gestation that exhibits any sign of life.

Secondary Outcome Measures
NameTimeMethod
Apgar score1 minute or 5 minutes after delivery

non

Serum vitamin levelsOne day before oocyte retrieval

Serum vitamin levels at the triggering day

Number of Participants with Clinical pregnancy4 weeks after embryo transfer

Clinical pregnancy: defined as at least one gestational sac on ultrasound at around 7 weeks' gestation with the detection of heart beat activity after the completion of the first embryo transfer;

Number of Participants with Ongoing pregnancyat 12 weeks' gestation

defined as pregnancy with detectable heart rate at 12 weeks' gestation or beyond after the completion of the first transfer

Number of available embryos for transfer5 days after oocyte retrieval

non

Number of Participants with Biochemical pregnancy2 weeks after embryo transfer

defined with a positive pregnancy test

Number of Participants with Pre-eclampsia20 weeks of gestation till delivery

defined as the development of gestational hypertension with proteinuria (≥300 mg/24-hour urine collection or 30 mg/dL in single urine sample) of new onset after 20 weeks of gestation after the completion of the first transfer

Number of Participants with Premature rupture of membraneThe day of delivery

PROM was defined as rupture of the amniotic membranes before the onset of labor including PROM at term and preterm PROM.

birth weightThe day of delivery

Weight of newborns at delivery.

Number of Participants with Cumulative live birth6 months after randomization of the last participant

Cumulative live birth: defined as live birth resulting from pregnancies that occur within 6 months after randomization.

Number of Participants with Ectopic pregnancy4 weeks after embryo transfer

defined as embryo implanted at any site other than the endometrial lining of the uterus cavity after the completion of the first transfer

Number of Participants with Gestational diabetes mellitusUp to ten months once the participants get pregnancy

defined as carbohydrate intolerance of variable severity with onset or first recognition during pregnancy as determined from the diagnosis in the obstetrical medical record

Number of Participants with Placenta previaafter 24 weeks of gestation

defined as a placenta that is implanted over or very close to the internal cervical orifice

Number of Participants with Stillbirth1 day after delivery

defined as the absence of signs of life at or after birth after the completion of the first transfer

Number of Participants with miscarriage24 gestational weeks in maximum

miscarriage was defined as a positive pregnancy test but no detectable heart rate before 24 weeks' gestation after the completion of the first transfer;

Number of Participants with Ovarian hyperstimulation syndromeFrom the day of oocyte retrieval to two weeks after embryo transfer

defined according to the Golan criteria. Moderate OHSS is diagnosed by the presence of ascites on ultrasonography in addition to abdominal distension and discomfort with or without nausea, vomiting and/or diarrhea. Severe OHSS is diagnosed when there is clinical evidence of ascites and/or hydrothorax or breathing difficulties with or without hemoconcentration, coagulation abnormalities and diminished renal function.

Number of Participants with Postpartum hemorrhage1 day after delivery

defined as the loss of 500 ml of blood or more after completion of the third stage of labor after the completion of the first transfer

Number of Participants with Preterm delivery1 day after delivery

Defined as delivery of a fetus at less than 37 and more than 28 weeks' gestational age after the completion of the first transfer

Number of Participants with Gestational hypertension20 weeks of gestation till delivery

defined as the development of blood pressure greater than 140/90 mmHg after pregnancy without proteinuria or other signs of preeclampsia after the completion of the first transfer;

Serum Vitamin D binding protein, glucose, insulin, Calcium ion concentrationThe day of oocyte retrieval

non

Trial Locations

Locations (2)

Peking University Third Hospital

🇨🇳

Beijing, Beijing, China

Women's Hospital, Zhejiang University School of Medicine

🇨🇳

Hangzhou, Zhejiang, China

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