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SARS-CoV-2 Antibodies Based IVIG Therapy for COVID-19 Patients

Phase 1
Completed
Conditions
COVID-19
Interventions
Biological: SARS-CoV-2 antibody based IVIG therapy
Registration Number
NCT04521309
Lead Sponsor
Dow University of Health Sciences
Brief Summary

Severe and critically ill patients will be enrolled in the study (50 patients) after duly filled consent forms. Recipients shall be divided in to 5 groups with 10 patients per group to compare clinical efficacy and safety of patients in clinical phase I/phase II study. Each group shall receive particular single dose of Intravenously administered Immunoglobulins (IVIG) developed from convalescent plasma of recovered COVID-19 individual , an experimental drug along with standard treatment except for control group which will receive standard treatment only.

Detailed Description

Passive immunization using intravenous immunoglobulins (IVIG) has been tested for treating previous viral outbreaks and holds the potential to save lives in the current crisis. Recently researchers from China reported satisfactory recovery of critically ill Corona Virus Disease 2019 (COVID 19) patients when high dose intravenous immunoglobulin (IVIG) were administered.

Research team at Dow University of Health Sciences has purified immunoglobulin (both SARS-CoV 2 antibodies and existing antibodies) from convalescent plasma of COVID19 individuals and pooled to prepared IVIG formulation to treat severe and critically ill COVID-19 patients. To evaluate safety of the formulation animal (rats) safety trials and survival of all the animals were observed.

It is intended to assess safety and efficacy of experimental the IVIG treatment in severe and critically ill COVID 19 patients through phase I/phase II randomized single blinded clinical trial with fifty study participants. FDA outlined criteria for passive immunization using convalescent plasma, which will be used for recruiting participants in the study.

Recruitment & Eligibility

Status
COMPLETED
Sex
All
Target Recruitment
50
Inclusion Criteria
  • Above 18 years of age
  • Have positive SARS-CoV-2 PCR on nasopharyngeal and/or oropharyngeal swabs
  • Admitted in isolation ward and ICU of institutes affiliated with DUHS
  • have severe or critical COVID 19 as judged by the treating physician
  • Consent given by the patient or first degree relative
Exclusion Criteria
  • Pregnancy
  • Previous allergic reaction to immunoglobulin treatment
  • Ig A deficiency
  • Patient requiring 2 inotropic agents to maintain blood pressures
  • Known case of any autoimmune disorder
  • Acute kidney injury or chronic renal failure
  • Known case of thromboembolic disorder
  • Aseptic meningitis

Study & Design

Study Type
INTERVENTIONAL
Study Design
SEQUENTIAL
Arm && Interventions
GroupInterventionDescription
IVIG dose: 0.20 g/kgSARS-CoV-2 antibody based IVIG therapyStandard Care + Single dose of 0.25 g/Kg anti-COVID19 IVIG (experimental drug prepared at DUHS) n= 10 patients
IVIG dose: 0.30 g/kgSARS-CoV-2 antibody based IVIG therapyStandard Care + Single dose of 0.35 g/Kg anti-COVID19 IVIG (experimental drug prepared at DUHS) n= 10 patients
IVIG dose: 0.25 g/kgSARS-CoV-2 antibody based IVIG therapyStandard Care + Single dose of 0.30 g/Kg anti-COVID19 IVIG (experimental drug prepared at DUHS) n= 10 patients
IVIG dose: 0.15 g/kgSARS-CoV-2 antibody based IVIG therapyStandard Care + Single dose of 0.20 g/Kg anti-COVID-19 IVIG (experimental drug prepared at DUHS) n= 10 patients
Primary Outcome Measures
NameTimeMethod
Adverse events during hospital stay28 days

Kidney failure, hypersensitivity with cutaneous or hemodynamic manifestations, aseptic meningitis, hemolytic anemia, leuko-neutropenia, transfusion related acute lung injury (TRALI)

Days to Hospital Discharge28 days

Duration from day of enrollment in study to Day of hospital discharge

Requirement of supplemental oxygen support28 days

Number of days required for invasive or non-invasive oxygen supply during hospital stay as per oxygen saturation status of patient

Change in neutrophil lymphocyte ratio28 days

change in neutrophil lymphocyte ratio from baseline will be used to monitor inflammation

Number of days on assisted ventilation28 days

Number of days a participant will be requiring assisted ventilation both invasive and noninvasive

28 Days mortality28 days

All cause mortality of participants will be monitored for 28 days to assess the safety and efficacy of IVIG treatment.

Days to step down28 days

Shifting from ICU to ward

Change in C-Reactive Protein (CRP) levels28 days

Change in C-Reactive Protein (CRP) levels from baseline will be used to monitor inflammation

Secondary Outcome Measures
NameTimeMethod
Change in radiological (X-ray) findings28 days

Any change seen in radiological chest X-ray findings

Change in Sodium levels28 days

Change in electrolytes (Sodium) seen in participants

Change in Chloride levels28 days

Change in electrolytes (Chloride) seen in participants

Change in fever28 days

Change in body temperature from baseline will be used to monitor safety and efficacy

Change in Ferritin levels28 days

change in Ferritin level from baseline will be used to monitor inflammation and immune dysregulation

Days to negative SARS-CoV-2 Polymerase Chain Reaction (PCR) test28 days

Time taken for participant to receive negative COVID-19 PCR test

Anti-SARS-CoV-2 Antibody28 days

Anti-SARS-CoV-2 antibody titre from blood measured by semi-qualitative method

Change in Potassium levels28 days

Change in electrolytes (Potassium) seen in participants

Change in Bicarbonate levels28 days

Change in electrolytes (Bicarbonate) seen in participants

Change in lactate dehydrogenase (LDH) levels28 days

change in LDH from baseline will be used to monitor infections and tissue health

Trial Locations

Locations (1)

Dow University of Health Sciences

🇵🇰

Karachi, Sindh, Pakistan

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