Investigating the Effects of Aerobic and Resistance Training in Vivo on Skeletal Muscle Metabolism in Vitro in Primary Human Muscle Cells (MoTrMyo)
Overview
- Phase
- N/A
- Intervention
- Not specified
- Conditions
- Physical Activity
- Sponsor
- AdventHealth Translational Research Institute
- Enrollment
- 400
- Locations
- 1
- Primary Endpoint
- Mitochondrial function
- Status
- Recruiting
- Last Updated
- last year
Overview
Brief Summary
The goal of the study is to examine the ability of resistance or aerobic exercise training to "imprint" skeletal muscle cells in a manner which confers long-term changes in this tissue which in-turn contribute to improved metabolic health and functional capacity through epigenetic regulation of novel exercise response genes. This study will also provide primary human skeletal muscle cells to the Molecular Transducers of Physical Activity Consortium (MoTrPAC) (NCT03960827) repository for future use.
Investigators
Eligibility Criteria
Inclusion Criteria
- •ADULT PARTICIPANT INCLUSION CRITERIA - SEDENTARY PARTICIPANTS
- •Willingness to provide informed consent to participate in the MoTrPAC Study
- •Willingness to undergo an extra needle insertion for the extra muscle tissue collection during the MoTrPAC muscle biopsy
- •Must be able to read and speak English well enough to provide informed consent and understand instructions
- •Aged ≥18 y
- •Body Mass Index (BMI) \>19 to \<35 kg/m2
- •Sedentary defined as self-reporting no more than 1 day per week, lasting no more than 60 minutes, of regular (structured) EE \[e.g., brisk walking, jogging, running, cycling, elliptical, or swimming activity that results in feelings of increased heart rate, rapid breathing, and/or sweating\] or RE (resulting in muscular fatigue) in the past year
- •Persons bicycling as a mode of transportation to and from work \>1 day/week etc. are not considered sedentary
- •Leisure walkers are included unless they meet the heart rate, breathing, and sweating criteria noted above
- •ADULT PARTICIPANT INCLUSION CRITERIA - HIGHLY ACTIVE PARTICIPANTS
Exclusion Criteria
- Not provided
Outcomes
Primary Outcomes
Mitochondrial function
Time Frame: Week 12 visit
Tested via a Analysis of Covariance (ANCOVA) with baseline as covariate followed by post-hoc multiple comparisons.
Insulin-stimulated glycogen synthesis in human skeletal muscle cell cultures (HSkMC)
Time Frame: Week 12 visit
Tested via a Analysis of Covariance (ANCOVA) with baseline as covariate followed by post-hoc multiple comparisons.