Targeting the beta-2 adrenergic pathway to improve skeletal muscle glucose uptake in healthy humans

Recruitment & Eligibility

Status: Completed

Sex: Not specified

Target Recruitment: 23

Inclusion Criteria

1. Caucasian;
2. Male sex;
3. Age: 18-30 years
4. BMI: 20-25 kg/m2;
5. Normal physical activity levels;

Exclusion Criteria

1. Not meeting all inclusion criteria
2. Cardiovascular disease (determined by means of questionnaires, heart
rate/blood pressure measurements and an ECG);
3. Respiratory diseases (including asthma, bronchitis and COPD);
4. Unstable body weight (weight gain or loss > 5 kg in the last three months);
5. Intention to lose or gain body weight (e.g. with caloric restriction or
physical activity)
6. Excessive alcohol and/or drug abuse;
7. Hypokalaemia;
8. Anaemia;
9. Epilepsy;
10. Smoking;
11. Renal and/or liver insufficiency;
12. Participation in another biomedical study within 1 month before the first
study visit, possibly interfering with the study results;
13. Medication use known to hamper subject*s safety during the study
procedures; *
14. Subjects who do not want to be informed about unexpected medical findings; *
15. Subjects who do not want that their treating physician to be informed;
16. Inability to participate and/or complete the required measurements;
17. Participation in organised or structured physical exercise;
18. Any condition, disease or abnormal laboratory test result that, in the
opinion of the Investigator, would interfere with the study outcome, affect
trial participation or put the subject at undue risk;
19. Hyperthyroidism

Study & Design

Study Type: Interventional

Study Design: Not specified

Primary Outcome Measures

Name	Time	Method
<p>Primary outcome parameter:<br /><br>- Insulin-stimulated peripheral glucose disposal (Rd) during the high-insulin<br /><br>infusion of the two-step hyperinsulinemic-euglycemic clamp</p><br>

Secondary Outcome Measures

Name	Time	Method
<p>Secondary study parameters:<br /><br>- Skeletal muscle GLUT4 translocation<br /><br><br /><br>Explorative objectives:<br /><br>- Body weight/composition<br /><br>- Plasma substrates<br /><br>- Heart rate and blood pressure<br /><br>- Insulin-mediated suppression of hepatic glucose production<br /><br>- (Sleeping) energy expenditure and substrate oxidation<br /><br>- Skeletal muscle glycogen and lipid content<br /><br>- Gene and protein expression in skeletal muscle<br /><br>- Femoral artery flow mediated dilation (FMD)</p><br>