Circulating microRNAs as Novel Prognostic Biomarkers in Obese Preschoolers at Risk for Type 2 Diabetes in Adulthood
Overview
- Phase
- Not Applicable
- Intervention
- Not specified
- Conditions
- Discriminate Obese Children at Risk for Diabetes.
- Sponsor
- Nanjing Medical University
- Enrollment
- 535
- Locations
- 1
- Primary Endpoint
- It is expected that the identification of circulating miRNAs as potential biomarkers may facilitate to the diagnosis of obese preschoolers at high risk for developing diabetes.
- Status
- Completed
- Last Updated
- 9 years ago
Overview
Brief Summary
Childhood obesity certainly increases susceptibility to type 2 diabetes (T2D) in adulthood. Recently, microRNAs (miRNAs) are closely related to various diseases and have been suggested as valuable biomarkers. Here, we sought to identify potential miRNAs to discriminate obese children at risk for diabetes in future.
Detailed Description
This study was designed to screen the circulating miRNAs in association with progression from children obesity to type 2 diabetes (T2D) in adulthood. Serum samples were evaluated in a 3 step procedure: 1) discovery study, in which samples from obese children and healthy controls were pooled for miRNA sequecing experiment; 2) cross-sectional validation study, in which miRNAs of interest were validated in all individuals (obese children, overweight children and normal controls); 3) longitudinal validation study, in which the candidate miRNAs were estimated in newly diagnosed diabetes patients and normal glucose tolerance controls (NGT). The final determined miRNAs were further confirmed though primary function studies.
Investigators
Xirong Guo
Vice-president
Nanjing Medical University
Eligibility Criteria
Inclusion Criteria
- •Obese preschoolers; Adults with type 2 diabetes; Age-matched healthy controls.
Exclusion Criteria
- Not provided
Outcomes
Primary Outcomes
It is expected that the identification of circulating miRNAs as potential biomarkers may facilitate to the diagnosis of obese preschoolers at high risk for developing diabetes.
Time Frame: 50-70 month old