Microbiome and Genetic Analysis of Familial IBD

Completed

• Conditions: Inflammatory Bowel Diseases

• Registration Number: NCT03515070
• Lead Sponsor: Kyunghee University Medical Center

Brief Summary

Inflammatory bowel disease(IBD) is a chronic inflammatory condition for gastrointestinal tract.

Regarding its pathogenesis, there has been numerous studies to reveal the complex association between genetic and environmental factors.

In Korea, the incidence of IBD is growing rapidly but genetic studies solely including patients with Korean descent were not sufficient enough.

Therefore, the investigators planned to conduct genetic and fecal microbial analysis for the 60 individuals from 30 Korean IBD families to find out the pathogenesis of IBD.

Detailed Description

Under the hypothesis that more risk variants will be observed among the familial IBD patient than in IBD patients without any other affected family members, the investigators designed genetic and fecal microbiome analysis for 60 patients form 30 families.

After extracting the DNA from blood samples, whole genome sequencing will be performed and data will be comprared with the previously reported variances. Novel variances or incidence of specific variances will be measured.

Genome-wide single nucleotide polymorphism array using Immunochip will be performed to search common genetic variants and to calculate genetic risk score of IBD.

In this study, fecal microbiome is a surrogate marker for the enviromental aspect of pathogenesis of IBD. The investigators assumed that family members are sharing similar mode of lifestyle therefore we're presumed that their fecal microbial composition is alike.

Comparing genetic and microbial datas altogether with the data from unaffected family member(Healthy internal control), investigators expecting to explain the genetic and enviromental aspect of IBD pathogenesis.

Study Timeline

• Study First Submitted: 2018-04-22
• Study First Submitted QC: 2018-04-22
• Study First Posted: 2018-05-03
• Study Start: 2018-05-09
• Primary Completion: 2019-09-30
• Study Completion: 2019-12-30
• Status Verified: 2020-02
• Last Update Submitted: 2020-02-18
• Last Update Posted: 2020-02-20

Recruitment & Eligibility

• Status: COMPLETED
• Sex: All
• Target Recruitment: 25

Inclusion Criteria

• 60 individuals from 30 families of Crohn's disease or ulcerative colitis.
• Unaffected 30 individuals from each family as healthy internal control.

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Exclusion Criteria

• Person with history of using antibiotics or probiotics within previous 4 weeks.

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Study & Design

• Study Type: OBSERVATIONAL
• Study Design: Not specified

Primary Outcome Measures

Name	Time	Method
Rare genetic variants of inflammatory bowel disease	Three months after the sample collection	Results from whole genome sequencing of blood samples of study participants. Planned to compare with the previously reported variances.
Genetic risk score of inflammatory bowel disease	Three months after the sample collection	Results from genome-wide single nucleotide polymorphism array of blood samples of study participants.; Planned to compare with the previously reported variances.
Common genetic variants of inflammatory bowel disease	Three months after the sample collection	Results from genome-wide single nucleotide polymorphism array of blood samples of study participants.; Planned to compare with the previously reported variances.
Fecal microbiome composition of each study subjects	Three months after the sample collection	Microbial diversity measured from 16S RNA sequencing datas of fecal microbiomes.

Trial Locations

Locations (1)

Kyung Hee University Medical Center; 🇰🇷 Seoul, Korea, Republic of